Poster Presentation

Poster Presentation

PR94 - The Effect of Lunasin on RANKL-Mediated Osteoclast Formation: An In-Vitro Analysis of Raw 264.7 Cells

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

Apical periodontitis (AP) is the destruction of apical periodontium which is mediated by host inflammatory responses. Interleukin-1 (IL-1) and tumor necrosis factor (TNF-a) are the primary stimulators of bone resorption in AP. Lunasin, a 43-amino acid soybean peptide, has been reported to reduce the levels of these inflammatory cytokines through inhibition of NF-kB pathway. No study has investigated the anti-resorptive properties of Lunasin. Therefore, the present study aimed to assess the effect of Lunasin on osteoclast formation using RAW 264.7 cells. We hypothesized that Lunasin is able to reduce the rate of osteoclast formation through inhibition of NF-kB. In the present in-vitro study, murine monocytic cell line (RAW-264.7) were differentiated into mature osteoclasts in the presence of recombinant RANKL. Tartrate-resistant acid phosphatase (TRAP) was used to identify mature osteoclasts. Activated osteoclasts were divided into different groups and exposed to 50 µM, 75 µM and 100 µM of active and inactive Lunasin. The positive control group consisted of RAW 264.7 cells treated with RANKL. TRAP positive cells with three or more nuclei were considered as mature osteoclasts by three independent observers. Data was analyzed using one-way ANOVA and Post-hoc Bonferroni (α=0.05). There was a significant reduction in the osteoclast formation in all the active Lunasin groups compared to the control and the inactive Lunasin group (P<0.001). Lunasin can exert an inhibitory effect on bone resorption by suppressing osteoclast formation. This may allow the potential use of lunasin to prevent the progression of AP and improve the outcome of endodontic interventions. 

Karishma R. Patil

Resident

I am currently a second year endodontic resident at Case Western Reserve University. I completed my dental school in 2014 from India and worked with an Endodontist before I started my residency at Case.

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Daisy Bachala

Endodontist
Case Western Reserve University

Daisy is a former resident at Case Western School of Dental Medicine. She graduated in 2017 June. She is now a practicing endodontist in Cleveland, Ohio.

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Nivine El-Refai

Volunteer Assistant Clinical Professor at Case Western Reserve University

Diplomate of the American Board of Endodontics
Volunteer Assistant Clinical Professor at Case Western Reserve University
Member of ADA, AAE, ODA, OAE
Vice President of Medina County Dental Society

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PR94 - The Effect of Lunasin on RANKL-Mediated Osteoclast Formation: An In-Vitro Analysis of Raw 264.7 Cells



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