Poster Presentation

Poster Presentation

PR52 - The Protective Role of N-Acetyl Cysteine With MTA and Bioceramic on Pulpal Stromal Cells Against Bacterial Infection

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

Vital pulp therapy (VPT) in response to carious pulp exposure involves either direct pulp capping or pulpotomy using Mineral Trioxide Aggregate (MTA) or Bioceramic (BC). MTA and BC are biocompatible cements with antibacterial activity. N-Acetyl Cysteine (NAC) is a membrane permeable amino thiol with anti-oxidant effects and induces Dental Pulp Stromal Cell (DPC) differentiation. However, the role of NAC on DPCs insulted with bacteria of pulpal infection has not been studied. We propose that NAC may have a synergistic effect with MTA or BC on DPCs insulted with bacteria in vitro. DPCs from extracted teeth were seeded onto MTA or BC. Cells were insulted with bacteria (Actinomyces or Fusobacterium nucleatum) or endotoxin (Lipopolysaccharide-LPS) with and without NAC. Flow Cytometry measured apoptosis (via propidium iodide staining) of DPCs and cell surface expression of Major Histocompatibility Complex (MHC) Class II. ELISA measured levels of IL6 and IL8 secretion. MTA and BC decreased apoptosis of DPCs insulted with bacteria or LPS. This protection was significantly potentiated with the addition of NAC. MHC Class II expression on DPCs and IL6 and IL8 secretion increased when cells were in contact with MTA plus NAC or BC plus NAC. Increase in MHC Class II and cytokines indicate NAC may play a role in recruiting immune effector cells (namely, T lymphocytes) along with neutrophil chemotaxis and angiogenesis. Synergism between NAC and MTA or BC increases anti-apoptosis. Our study brings important implications of the potential use of NAC to protect DPCs affected by carious bacteria in VPT.

Divya Kanodia

Resident

I, Divya Kanodia graduated from Government Dental College and Hospital, Mumbai with a Bachelors in Dental Surgery Degree in 2015. My particular interest in Endodontics lead me to pursue the same at University of California, Los Angeles as an Advance Clinical Trainee. I have been working with Dr. David Han and Dr. Anahid Jewett on our research project, "The Protective role of NAC with MTA/BC putty in vital Pulp Therapy." I have a keen interest in delving deeper into this field of study and have been really enjoying working on this topic.

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David C. Han

Clinic Director, Post-Graduate Endodontics
University of California, Los Angeles

I am a Diplomate of the American Board of Endodontics and currently balance my time between private practice and teaching at UCLA School of Dentistry, Section of Endodontics, 50%. I own two private practices limited to endodontics in southern California with four endodontists working at both locations. I am also Clinic Director in the Post-Graduate Endodontics Department at UCLA School of Dentistry. I have always had a passion for research in immunology and bacteriology, but have been busy with private practice in the past. The last three years, however, I have had the opportunity to teach more significantly and have connected with my previous PI, Dr. Anahid Jewett, who is excited to collaborate with me and the residents I mentor. Regenerative endodontics has also been of high interest to me and there is a pending IRB application that I am submitting for clinical trials. My goal is to continue to mentor residents in research that bridges the gap between basic and clinical sciences, namely immunology and endodontics

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Anahid Jewett

Professor and Director of Tumor Immunology Laboratory
University of California, Los Angeles

I have a long track record of the studies of immune cells in particular NK cells and monocytes, DCs, and osteoclasts in mice and human in health and disease. I also have significant experience in studying T cells. We have recently discovered that NK cells target both untransformed healthy stem cells as well as cancer stem cells, therefore NK cells are very important in limiting the size of the stem cells and they play a key role in the maturation and differentiation of stem cells. I have the skills, expertise, leadership and motivation required to successfully carry out the proposed studies. I have a broad background in Immunology, with specific training and expertise in Tumor Immunology, toxicology and drug related studies. Indeed, this work is perfect marriage of two important and key areas of on-going studies in my laboratory. I have more than 20 years of experience in conducting focused research in the fields of NK cell immuno-biology and drug related studies in cancer. I have a demonstrated record of successful and productive research projects in the area of tumor immunity, and my expertise and experience have prepared me well to partake in the proposed project.

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PR52 - The Protective Role of N-Acetyl Cysteine With MTA and Bioceramic on Pulpal Stromal Cells Against Bacterial Infection



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