Poster Presentation

Poster Presentation

PR59 - The Role of WDR72 in the Secretion of the Enamel Protease Kallikrein-4 During Amelogenesis

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

Amelogenesis Imperfecta (AI) is a rare genetic disease affecting dental enamel formation. Patients with AI suffer from dental and esthetic problems. There are two stages to enamel formation; secretory and maturation stages. During the maturation stage, hydroxyapatite deposition and mineralization occur leading to the normal, hard layer of enamel. AI with WDR72 mutations have weak and soft enamel characteristics similar to mutations that happen during the maturation stage of amelogenesis. During maturation, ameloblasts secrete kallikrein-4 (KLK4) responsible for extra-cellular matrix degradation leading to subsequent inorganic deposition and mineralization. In this study, we hypothesized that WDR72 plays a role in the secretion of KLK4. The aims of this study were to investigate the stage at which WDR72 is expressed during amelogenesis and to investigate whether WDR72 has an effect on the secretion of KLK4. Immunohistochemistry with anti-WDR72 antibody was performed on WDR72 wild-type and knock-out rats to examine the expression of WDR72 during secretory and maturation stages of amelogenesis. Mouse ameloblasts were used to co-transfect WDR72 and KLK4 to examine the effects WDR72 on KLK4 secretion. Our immunohistochemical staining data demonstrated that WDR72 expression was positive only in the wild type ameloblasts of rats during the maturation stage of amelogenesis. Our data also showed that extracellular KLK4 localization was increased when WDR72 was co-transfected. In conclusion, our results demonstrate that WDR72 is mainly expressed in the cytoplasm of ameloblasts at the maturation stage and that WDR72 may be involved in the secretory pathway of KLK4.

Dina R. Husein

Endodontic resident
Boston University

Graduated in 2010, practiced for two years. Had a cranio-facial fellowship from Nova Southeastern University in 2014. A doctorate degree candidate 2014-17, Endodontic resident 2017-19

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Yoshiyuki Mochida

Clinical Assistant Professor, Department of Molecular & Cell Biology

Yoshiyuki Mochida, DDS, Ph.D. Graduated from Japan
A Clinical Assistant Professor at Boston University
Henry M. Goldman School of Dental Medicine
Department of Molecular and Cell Biology

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PR59 - The Role of WDR72 in the Secretion of the Enamel Protease Kallikrein-4 During Amelogenesis



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