Poster Presentation

Poster Presentation

PR62 - The Use of Three-Dimensional Sphere Forming Cells Towards Pulp Revascularization

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

Objective: Pulp vascularization is essential for successful pulp regeneration. Dental pulp stem cells (DPSCs) are known to contribute to pulp vascularization by 1) differentiating into endothelial cells and 2) secreting pro-angiogenic factors in pulp microenvironment, thereby promoting vascularization. Recently, we reported the presence of 3D sphere-forming stem cell-like subpopulation (DPSC spheres) in DPSCs and their enhanced multi-lineage differentiation potential.  However, their application for pulp vascularization is not explored. In this study, we tested the potential usage of DPSC spheres in pulp vascularization by investigating their endothelial differentiation potential and their expression of pro-angiogenic factors compared to control DPSCs. Methods: Primary DPSCs were isolated from extracted human teeth, and DPSC spheres were collected by sphere formation assay. Endothelial differentiation potential of DPSC spheres and DPSCs was tested by measuring key transcription factors that regulate endothelial cell development. The level of pro-angiogenic factors was determined by quantitative RT-PCR. Results: DPSC spheres were enriched with the key transcription factors for endothelial differentiation, including Kruppel-like factor (KLF). The expressions endothelial differentiation markers were also increased in DPSC spheres.  Moreover, DPSC spheres showed elevated expression of pro-angiogenic factors (e.g., VEGF, RANTES) compared to DPSCs. Conclusions: DPSC spheres harbor enhanced pro-angiogenic ability, suggesting that DPSC spheres may provide unique opportunity for pulp vascularization. Further studies will need to be conducted in order to demonstrate enhanced differentiation potential of DPSC spheres and increased secretion of the pro-angiogenic factors from DPSC sphere. 

Deepika Ganesh

Endodontic resident
University of California, Los Angeles

I am currently a 2nd year resident in the Section of Endodontics at the School of Dentistry, UCLA. I completed my Dental education in India in 2010 at D.Y Patil Dental College and Hospital, Navi Mumbai. I practised as a general dentist for 3.5 years before I embarked on chasing my aspiration of becoming an Endodontist. I have been working on the project titled " The use of three-dimensional sphere forming cells towards pulp revascularization" under the mentorship of Dr. Ki-Hyuk Shin. I have been working under Dr. Shin’s guidance for 2 years at UCLA and accumulated sufficient preliminary data to propose current study. My past experiences include working on projects studying “a novel approach to pulp tissue engineering using 3 dimensional spheres derived from dental pulp stem cells (DPSCs)” and “the roles of cytokines on stemness of DPSCs”. My experience and knowledge will be appropriate to conduct the proposed studies as PI under Dr. Shin’s guidance

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Sung Hee Lee

Postdoctoral Fellow

I am currently a post-doc fellow in the Oral Biology Division at the University of California, Los Angeles. I have been investigating molecular determinants regulating stemness properties of normal and cancer stem cells. Recently, our research group reported for the first time the role of Orai1 calcium channel on stem cells. Specifically, under the mentorship of Dr. Ki-Hyuk Shin, I shall take the role as co-investigator with Dr. Ganesh.

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Nadia Chugal

Program Director
University of California at Los Angeles

Dr. Nadia Chugal, D.D.S., M.S., M.P.H., is the Program Director for the Postdoctoral Residency Program in Endodontics and Clinical Professor at the UCLA School of Dentistry. She joined the Dental School as the faculty in 1997. Dr. Chugal is known for her expertise in program administration, innovation and excellence in education, leadership and professionalism. She has held leadership positions in local, national and international professional organizations. Dr. Chugal is the past president of the Southern California Academy of Endodontics, (SCAE) and has been Secretary and Treasurer for the Pulp Biology Group (PBRG) of the International Association for Dental Research (IADR) since 200

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Mo K. Kang

Professor, Chair of the Division of Constitutive and Regenerative Sciences, Chair of the Section of Endodontics, Jack A. Weichman Chair in Endodontic
University of California, Los Angeles

Mo Kwan Kang, M.S., Ph.D., D.D.S. is a professor and the chair of the Section of Endodontics and the Division of Constitutive & Regenerative Sciences at the UCLA School of Dentistry. Dr. Kang also holds a dual appointment with the Section of Oral Biology and is the first person to hold the Dr. Jack A. Weichman Chair in Endodontics. The current research in Dr. Kang’s laboratory is focused on oral mucosal diseases, including oral cancer, chemical- and radiation-induced oral mucositis, and aging-associated oral epithelial dysfunction. These studies were developed in his laboratory from extensive investigations of the mechanisms underlying cellular senescence (aging) and immortalization of normal human oral keratinocytes (NHOK)

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Ki-Hyuk Shin

Adjunct Professor

Dr. Ki-Hyuk Shin is an adjunct professor in the Division of Oral Biology and Medicine at the UCLA School of Dentistry. He is an active member of Jonsson Comprehensive Cancer Center at the David Geffen School of Medicine at UCLA. Dr. Shin’s' research program is founded on the question of how to identify and functionally define genetic and epigenetic alteration during oral carcinogenesis. This central theme has reached over time into three related areas: 1) the role of aberrant DNA repair activities in oral cancer; 2) the role of Human Papillomavirus in oral cancer; 3) the role of epigenetic regulators in oral cancer; 4) the understanding of molecular characteristics of oral cancer stem cells; and 5) the mechanism of senescence in human oral keratinocytes

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