Poster Presentation

Poster Presentation

PR71 - Efficacy of Different Chitosan Nanoparticle Combinations in Inhibiting Growth of Two Strains of Enterococcus Faecalis

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

Objectives: This study aimed to investigate the antimicrobial efficacy of chitosan nanoparticles (CS-np), CS-np loaded with curcumin (CS-np/C) and CS-np with Tween 80 loaded with curcumin (CS-np/C/T80). Methods: CS-np were prepared, their particle size and zeta potential characterized, and functional integrity of the developed formulations confirmed. Two Enterococcus faecalis strains (ATCC 29212 and 33186) were incubated in brain-heart infusion (BHI) to reach 108 cells/mL. BHI/agar plates were inoculated with either strain separately. Three formulations of CS-np combinations were prepared aseptically and tested with the agar disk-diffusion method: 1) CS-np, 2) CS-np/C and 3) CS-np/C/T80 each at concentrations of 2, 5 and 10 mg. BHI disks were used as controls. Petri dishes were incubated at 37°C for 24 hours and the diameters of the zones of inhibition measured to the nearest mm. All tests were performed in triplicate. Data were analyzed using a two-way ANOVA and Tukey’s multiple comparison test (α<0.05). Results: All compounds and concentrations showed antimicrobial activity when compared to the control (p<0.001). The susceptibility of the microorganisms against each compound varied. CS-np/C was the least effective combination (p<0.01) against ATCC 29212 at 2 and 10 mg when compared to CS-np and CS-np/C/T80, which revealed the highest antimicrobial effects (p<0.01). For ATCC 33186, CS-np/C/T80 showed the highest zone of inhibition at 5 and 10 mg when compared to CS-np and CS-np/C, which showed the lowest (p<0.01). Conclusion: The addition of both curcumin and Tween 80 to chitosan nanoparticles had stronger antibacterial activity against Enterococcus faecalis than adding curcumin alone.

Michael A. James

Endodontic Resident
University of Tennessee

Received a Master's in Public Health from Hunter College SUNY in Manhattan, in 2002. Graduated from the University of Tennessee Health Science Center in 2013. Moved to Bronx New York for a 2 year GPR Residency at Montefiore Hospital (2013-2015) and was the chief resident during his second year. Is currently a second year Endodontic Resident at the University of Tennessee Health Science Center.

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Pallabita Chowdhury

Graduate Assistant / Department of Pharmaceutical Sciences
University of Tennessee

Research experience
Aug 2013– Jun 2015: Graduate Assistant, University of Toledo · Department of Pharmacy Practice, United States · Toledo
Current: Graduate Assistant, University of Tennessee Health Science Center, Department of Pharmaceutical Sciences.
In addition, she is currently a third year PhD student

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Murali M. Yallapu

Assistant Professor / Pharmaceutical Sciences
University of Tennessee

Postdoc/Staff Scientist, Sanford Research/USD, Nanoparticles and Drug Delivery
PostDoc, Cleveland Clinic, Nanoparticles and Drug Delivery
PostDoc, University of Nebraska Medical Center, Nanoparticles and Drug Delivery
PostDoc, Gwangju Institute of Science & Technology, South Korea, Materials Science, Hydrogels and Nanoparticles
Ph.D., Sri Krishnadevarya University, Polymer Science

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Adam Lloyd

Associate Professor and Chair Department of Endodontics
University of Tennessee

Dr. Adam Lloyd received his dental degree from Cardiff Dental School in 1994, followed by completion of an endodontic residency and masters in oral biology at Baylor College of Dentistry, Dallas, Tex. Dr. Lloyd taught at the University of California San Francisco and NSU College of Dental Medicine before his appointment as associate professor at the University of Tennessee Health Science Center College of Dentistry. He is currently the chair of the department of endodontics and the program director of the Advanced Specialty Education Program in Endodontics, having founded the residency program and built a state of the art facility in Memphis. Dr. Lloyd has received awards from the British Endodontic Society, including the Fred Harty medal and most recently has been recognized as an award winning educator by the American Association of Endodontists, receiving the 2010 Edward M. Osetek Educator award, the 2010 UTHSC SGAEC Excellence in Teaching Award and the faculty of the year award by the graduating class of 2015. At UTHSC his research is based on x-ray micro-focus computerized tomography and imaging modalities for canal preparation, obturation and disinfection. He has published in the endodontic literature and lectured internationally.

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Josanne M. O'Dell

Assistant Professor / Department of Endodontics
University of Tennessee

J.D., Trinity Law School, Santa Ana, Calif., Law
None, Loma Linda, Loma Linda, Calif., Implantology
None, University of Southern California, Los Angeles, Calif., Certificate, Endodontics D.D.S., University of Colorado, Denver, Colo., Dentistry
M.F.S., George Washington University, D.C., Forensic Science

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Jegdish P. Babu

Professor Bioscience Research
University of Tennessee

Associate Professor, Department of Bioscience Research, UTHSC College of Dentistry
Scientific Training: Osmania Univ., India (M.Sc.); West Virginia Univ. (MS); Michigan State Univ. (PhD – Microbiology & Immunology)
Academic Responsibilities: basic & clinical science and MDS research

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Franklin Garcia-Godoy

Senior Executive Associate Dean for Research / Department of Bioscience Research
University of Tennessee

Dr. Franklin Garcia-Godoy is the Senior Executive Associate Dean for Research, Director of the Bioscience Research Center, and Chair, Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, Tennessee. He has published over 525 scientific articles and collaborates with researchers located in universities all over the world and over the years has helped train and educate hundreds of dentists who serve communities across the U.S. and all over the world. At a national level, Dr. Garcia-Godoy was a member of several National Institutes of Health review panels and was on the National Advisory Board to the Director of the National Institute of Dental and Craniofacial Research (2008-2012). He is also in the advisory boards of several large companies. Dr. Garcia-Godoy is the Editor of the American Journal of Dentistry, Associate Editor, Journal of Dentistry, Associate Editor, Stem Cells and Development, and Associate Editor, Current Tissue Engineering. He received his D.D.S. from the Autonomous University of Santo Domingo, a Certificate and M.S. in Pediatric Dentistry at the University of Illinois at Chicago, a Ph.D. in Biotechnology from University of Siena, and a PhD in Dental Materials from the University of Amsterdam (ACTA) .

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Subhash C. Chauhan

Professor Pharmaceutical Sciences
University of Tennessee

Primary research interest of Dr. Chauhan’s lab is to identify and characterize the diagnostic and therapeutic targets for cancer, such as ovarian cancer, cervical cancer, prostate cancer, pancreatic cancer. This research is aimed for the identification and characterization of biomarkers that aberrantly express or localize in cancer cells in order to develop newer tools for early disease diagnosis. Recently we have identified a novel trans-membrane mucin MUC13 which is highly over-expressed ovarian and pancreatic cancer cells. This may be potential biomarker for early cancer diagnosis as well as a good target for antibody guided targeted cancer therapy.

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George TJ. Huang

Professor and Director Bioscience Research
University of Tennessee

PostDoc, UC San Diego, Mucosal Immunology
D.Sc., Boston University, Oral Biology
M.S.D., Boston University, Endodontics
Certificate, Boston University, Endodontics
D.D.S., Taipei Medical College, Dentistry
Published a number of highly cited review papers retrospectively outlined the important progress in the field of pulp regeneration and prospectively discussed what remains to be investigated in order to launch stem cell-based pulp regeneration in humans. In addition, established a tooth fragment model using SCID mice to study pulp regeneration which has since been used by many researchers in the field, we also established a large animal model using mini-swine to investigate pulp and dentin tissue regeneration. In terms of publications, I have published (+ in press) close to 70 peer reviewed articles and 17 book chapters as well as co-edited with Dr. Irma Thesleff a ground breaking textbook “Stem cells in craniofacial development and regeneration” Wiley & Blackwell, 2013.

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Melissa A. Marchesan

Associate Professor / Department of Endodontics
University of Tennessee

Dr Melissa A. Marchesan graduated dental school from the University of Sao Paulo in 1998. She pursued a Masters in Restorative Dentistry with focus in Endodontics and Lasers at the University of Sao Paulo. During 6 years Dr Marchesan engaged in teaching predoctoral and postgraduate students at the University of Ribeirao Preto (UNAERP) in Brazil. In 2007, she completed her PhD in Dentistry furthering her research in Endodontics and Lasers. From 2010 to 2014, Dr Marchesan held a position as an Associate Professor at Nova Southeastern University, Department of Endodontics and had a roll in predoctoral teaching and mentoring Graduate Endodontic resident research. In 2011, she received the Endodontic Educator Fellowship Award from the American Association of Endodontics and redid her Endodontic specialty in the United States. In 2014, she was recruited by the University of Tennessee Health Science Center, College of Dentistry, Department of Endodontics for an Associate Professor position. At which she develops research, mentors postgraduate Endodontic Residents and collaborates in predoctoral and postdoctoral teaching. She was appointed to represent the Resident and New practitioners on the American Association of Endodontists Foundation Board of Trustees.

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PR71 - Efficacy of Different Chitosan Nanoparticle Combinations in Inhibiting Growth of Two Strains of Enterococcus Faecalis



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