Poster Presentation

Poster Presentation

PR108 - The Effect of Calcium Silicate-Based Cements on Osteoclastic Activity Using Murine Bone Marrow Macrophage

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

Objectives: Biodentine (BIOD), one of the calcium silicate-based cements (CSCs) is a new bioactive repair and dentine-substitute containing similar components to mineral trioxide aggregate (MTA). The purpose of this study is to evaluate the effect of BIOD and MTA on the osteoclast (OCLs) derived from mouse bone marrow macrophages (BMMs), and compare it to that of alendronate. Materials and Methods: Murine BMMs were induced to differentiate into OCLs with M-CSF and receptor activator of NF-kB (RANKL) followed by lipopolysaccharide (LPS). After Biodentine (BIOD), ProRoot MTA (PMTA) or alendronate (ALD) treatment, cytotoxicity was determined with WST-1 assay, and RANKL/LPS-induced osteoclast differentiation and function were assessed tartrate-resistant acid phosphate (TRAP) staining and resorption pit assay. The mRNA expression involved in osteoclastic activities was detected with quantitative real-time polymerase chain reaction (qRT-PCR). Related molecular signaling pathways were investigated with Western blot. Data were analyzed by one-way ANOVA and Tukey’s post-hoc test. Results: Murine BMMs treated with BIOD, PMTA or ALD showed significantly depressed levels of TRAP positive OCLs (p<0.05). BIOD treatment inhibited mRNA expression of TRAP, Cathepsin K, and c-Fos compared to OCLs, however, showed less potent than PMTA and ALD treatment (p<0.05). BIOD and PMTA decreased RANKL-mediated phosphorylation of ERK1/2 and IkBa comparable with ALD. Conclusions: Calcium silicate-based cements including Biodentine and ProRoot MTA exhibit comparable inhibitory effect to alendronate on osteoclast differentiation and functions via inhibition of ERK signaling pathways as well as IkB phosphorylation.

TaeUk Sun

Resident

I graduated Segwang high school and Dankook University in Cheonan, Republic of Korea. Now, I'm now resident of the preservative dentistry in Dankook Dental Hospital in Korea.

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Miri Kim

Associate Professorate Professor
Ulsan University, Asan Medical Center

Associate Professor in Dentistry, UUCM AMC
Visiting Scholar at University of Pennsylvania, USA
Assistant Professor in Dentistry, UUCM AMC
Fellowship in Dentistry, UUCM AMC Ph.D. in Dental Science : Seoul National University
Master of Dental Science : Seoul National University
Bachelor of Dentistry : Seoul National University

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Hyunjung Ko

Associate Professor
Ulsan University, Asan Medical Center

Ph.D. in Dental Science : Seoul National University
Master of Dental Science : Seoul National University
Bachelor of Dentistry : Seoul National University Assistant Professor, Associate Professor in Dentistry, UUCM AMC
Postdoctoral research fellow, Univ.of Pennsylvania, USA
Postdoctoral research fellow, Univ.of North Carolina,USA

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Minju Song

Assistant professor
Dankook University

2007.03-2010.02 Resident, Department of Conservative Dentistry, Dental Hospital of Yonsei University
2010.03-2013.02 Clinical research assistant professor, Department of Conservative Dentistry, Gangnam Severance Dental Hospital, College of Dentistry, Yonsei University
2013.03-2014.12 Clinical assistant professor, Department of Conservative Dentistry, Gangnam Severance Dental Hospital, College of Dentistry, Yonsei University
2015.01- 2017.01 Visiting Assistant Project Scientist, Section of Restorative Dentistry, Division of Constitutive & Regenerative Sciences, UCLA School of Dentistry
2017.02-presnet Clinical assistant professor, Department of Conservative Dentistry, Dental Hospital, College of Dentistry, Dankook University

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PR108 - The Effect of Calcium Silicate-Based Cements on Osteoclastic Activity Using Murine Bone Marrow Macrophage



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