Poster Presentation

Poster Presentation

PR20 - Immunohistochemical Analysis of Pulpal Vascular and Neural Tissue in a Pig Endodontic Regeneration Model

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

Introduction: This study used immunohistochemistry to evaluate early revascularization and nerve formation following therapies utilizing BiodentineTM (Septodont, Saint Maur des Fosses, France) and ProRoot MTATM (Dentsply Sirona, Tulsa, OK) in a young pig model for endodontic regeneration. Methods: Routine immunohistochemical techniques were used to characterize cell populations and mineralized tissues in samples previously obtained from a study utilizing a young pig model for endodontic regeneration, in which mandibular first and second premolars in six pigs (three-months-old) received regenerative treatment. Teeth were divided into five treatment groups: blood clot and MTA; blood clot and Biodentine; blood clot without bioceramic; MTA without blood clot (positive control); and no treatment (control). Samples were harvested after a three-week healing period, fixed in formalin, evaluated radiographically, and processed for histology. Sections were immunohistochemically stained for markers of blood vessels and nerves including Griffonia simplicifolia lectin, alcam, endoglin, actin, ubiquitin hydrolase, calcitonin gene-related peptide, and tyrosine hydroxylase. Results: The blood clot treatment groups demonstrated extensive areas of new vessel formation adjacent to active hard tissue matrix formation on the canal walls while these new vessels were absent in areas in which no active deposition of hard tissue matrix was observed. Prominent nerve formation within the pulp was not evident at three weeks. Conclusion: Revascularization appeared to correlate with new hard tissue formation in the apical root region following endodontic regenerative treatment. Pulpal innervation, if any, may occur later. The young porcine model shows promise for characterization of revascularization via immunohistochemistry.

Steve Johnson

CPT
US Army Dental Activity, Ft Gordon

Graduated dental school at University of Southern California. Graduated 1 year AEGD at Joint base Lewis-McCord. Currently a second year endodontics resident at the Fort Gordon Endodontics residency.

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Douglas Dickinson

Dental Research Analyst
US Army Dental Activity, Ft Gordon

Dr. Douglas Dickinson has worked as a dental research analyst at Fort Gordon US Army Dental Activity for 5 years. Previously, he worked for Augusta University as a dental research analyst. He retired this past summer.

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Stephanie Sidow

Residency Director
US Army Dental Activity, Ft Gordon

Dr. Stephanie Sidow graduated from Marquette School of Dentistry. She has been the Residency Director of the Advanced Education Training in Endodontics at Fort Gordon for 1 and 1/2 years.

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Joseph Dutner

Assistant Residency Director
US Army Dental Activity, Ft Gordon

Dr. Joseph Dutner graduated from Temple University School of Dentistry. He has been the assistant director of the US Army Advanced Education Program in Endodontics at Fort Gordon for 1 and 1/2 years.

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Joshua Pruden

Endodontic Resident
US Army Dental Activity, Ft Gordon

Dr. Joshua Pruden attended West Virginia School of Dentistry and graduated in 2012. He served in the US Army as general dentist for four years. He is currently in his 2nd year of residency at the Fort Gordon Endodontic Residency in Augusta, GA. He has been married for 7 years and has four children.

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