Poster Presentation

Poster Presentation

PR33 - Immunohistochemical Phenotype Analysis of Cells Associated With Pulpal Mineralized Extracellular Matrix in a Pig Endodontic Regeneration Model

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

Objectives: The aims of this study were to evaluate the young pig as a model for the study of endodontic regenerative treatment and to compare via immunohistochemistry the early cellular and mineralizing events occurring at the apex, following therapies utilizing BiodentineTM (Septodont, Saint Maur des Fosses, France) and ProRoot MTATM (Dentsply Sirona, Tulsa, OK). Methods: Routine immunohistochemical techniques were used to characterize cell populations and mineralized tissues in samples previously obtained from a study utilizing a young pig model for endodontic regeneration, in which mandibular first and second premolars in six pigs (3-months old) received regenerative treatment. Teeth were divided into five groups, treated with: blood clot and MTA (group one); blood clot and Biodentine (group two); blood clot without bioceramic (group three); MTA without blood clot (group four, positive control); and no treatment control (group five). Samples were harvested after a three-week healing period, fixed in formalin, evaluated radiographically, and processed for histology. Sections were immunohistochemically stained for mineralized matrix markers including dentin sialophosphoprotein, osteocalcin, osteopontin, cementum-attachment protein, osterix, runt-related transcription factor 2, and proliferating cell nuclear antigen. Results: The blood clot treatment groups demonstrated extensive areas of active hard tissue matrix formation on the canal walls resembling cementum-like deposition extending from the apex with high levels of cementoblast-like cell activity. Conclusion: A cementum-like mineralization can occur in the apical root region following endodontic regenerative treatment. The porcine model shows promise for the study of these events.

Joshua Pruden

Endodontic Resident
US Army Dental Activity, Ft Gordon

Dr. Joshua Pruden attended West Virginia School of Dentistry and graduated in 2012. He served in the US Army as general dentist for four years. He is currently in his 2nd year of residency at the Fort Gordon Endodontic Residency in Augusta, GA. He has been married for 7 years and has four children.

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Douglas Dickinson

Dental Research Analyst
US Army Dental Activity, Ft Gordon

Dr. Douglas Dickinson has worked as a dental research analyst at Fort Gordon US Army Dental Activity for 5 years. Previously, he worked for Augusta University as a dental research analyst. He retired this past summer.

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Stephanie Sidow

Residency Director
US Army Dental Activity, Ft Gordon

Dr. Stephanie Sidow graduated from Marquette School of Dentistry. She has been the Residency Director of the Advanced Education Training in Endodontics at Fort Gordon for 1 and 1/2 years.

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Joseph Dutner

Assistant Residency Director
US Army Dental Activity, Ft Gordon

Dr. Joseph Dutner graduated from Temple University School of Dentistry. He has been the assistant director of the US Army Advanced Education Program in Endodontics at Fort Gordon for 1 and 1/2 years.

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PR33 - Immunohistochemical Phenotype Analysis of Cells Associated With Pulpal Mineralized Extracellular Matrix in a Pig Endodontic Regeneration Model



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