Poster Presentation

Poster Presentation

PR31 - Single Nucleotide Polymorphisms in β-catenin Destruction Complex Genes: Potential Role in Apical Periodontitis Development

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

Objective: Wnt/β-catenin signaling attenuates periapical bone destruction suggesting potential role for these proteins in apical periodontitis (AP) development. At the heart of the canonical Wnt signaling pathway is the β-catenin destruction complex (AXIN, GSK3B, APC). We hypothesized that single nucleotide polymorphisms (SNPs) in these genes may lead to altered gene expression and contribute to susceptibility to AP development. Methods: Cases (n=185) were subjects recruited at UTHealth School of Dentistry showing deep carious lesions and AP lesion (≥3 mm); whereas controls (n=160) were subjects with deep carious lesions but without AP lesion. Saliva samples were used as source of genomic DNA. Twenty-two SNPs in/nearby the β-catenin destruction complex genes AXIN1, AXIN2, APC, GSK3B and CTNNB1 were genotyped using Taqman chemistry in real-time PCR. Allele and genotype frequencies between cases and controls were assessed using chi-square and Fisher's exact tests as implemented in PLINK vs.1.06. Bonferroni method was applied to correct for multiple testing. mRNA expression of these genes was assessed in 93 human periapical granulomas and 24 healthy periodontal ligament tissues using SYBR green chemistry in real time PCR. Results: Significant genotypic association was found between CTNNB1 rs1880481 and cases with deep caries and AP lesions (p=0.03). Expression of AXIN1, AXIN2, APC, GSK3B and CTNNB1 was significantly higher in periapical granulomas in comparison to healthy tissues (p< 0.001). Conclusion: Variations in β-catenin destruction complex genes may be associated with periapical lesion predisposition in individuals with untreated deep caries. Future studies could help predict host susceptibility to developing AP.

Ahmad-Reza Noroozi

Endodontic Resident (2nd Year)
University of Texas at Houston

I am currently a second-year endodontic resident at the University of Texas in Houston. I also have completed a prior residency in oral and maxillofacial surgery in Bronx, NY and am a Board certified oral surgeon in both the United States and Canada.

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Josh Johnson

2nd year DDS student

Second year DDS student at School of Dentistry, University of Texas Health Science Center at Houston. Josh was sponsored by UT School of Dentistry Summer research program

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Letícia Chaves de Souza

Postdoctoral Research Fellow
University of Texas at Houston

Dr. Leticia Chaves de Souza obtained her DDS degree in 2004 at the Federal University of Rio de Janeiro, Brazil, followed by a Residency in Endodontics at the same University, MS in Endodontics at Estácio de Sá University and Ph.D. in Materials Science in Brazil. Her research areas of interest are endodontic biomaterials, endodontic instruments, root canal disinfection protocols and cellular and molecular basis of apical periodontitis. Currently a postdoctoral research fellow at the University of Texas in Houston doing research in the endodontic department focus on genetic predisposition to apical periodontitis.

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Bruno Crozeta

PHd Student
University of Texas at Houston

Graduated in Dentistry from the University of Joinville Region - UNIVILLE (2012). Master in Clinical / Endodontics and Dental Specialist in Endodontics Positivo University - Brazil. Is currently a PhD student in Endodontics, School of Dentistry of Ribeirão Preto – University of São Paulo and University of Texas of Houston Health Science at Houston – USA . As a student researcher, operates in the following areas and subareas: dentistry, endodontics, endodontic retreatment, endodontic instruments , ultrassonics, dental anatomy and cellular and molecular basis of apical periodontitis

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Leticia Guajardo

Assistant Professor

Dr. Guajardo is a DDS/endodontist and currently an Assistant Professor at School of Dentistry, Department of Endodontics University of Texas health Science center at Houston.

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Gustavo P. Garlet

Associate Professor & Head - Dept of Biological Sciences
University of São Paulo

Dr. Garlet is a DDS/PhD and an Associate Professor & Head - Dept of Biological Sciences and Osteoimmunology Laboratory at School of Dentistry of Bauru - FOB/USP

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Ariadne Letra

Associate Professor
University of Texas at Houston

Dr. Letra is a DDS PhD with extensive clinical and research expertise in dental sciences. Her research interests rely on genetics of complex oral traits in humans and understanding the biological mechanisms underlying genetic variations in vivo and in vitro.

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Renato Silva

Associate Professor and Chair
University of Texas at Houston

Dr. Silva is an Associate professor and chairman, Department of Endodontics at University of Texas Health Science Center at Houston. His research focuses on understanding the molecular mechanisms underlying wound healing in chronic inflammatory diseases of the jaws.

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