Poster Presentation

Poster Presentation

PR49 - Biocompatibility of MTA Mixed with Human Platelet Lysate

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

Objective: The overall goal of this work is to understand the potential role of platelet rich plasma in regenerative endodontic therapy. The aim of this study was to evaluate the biocompatibility of gray ProRoot MTA Root Canal Repair Material mixed with human platelet lysate (HPL) compared to the same MTA material mixed with distilled water. Materials and methods: In these in vitro study, biocompatibility of human osteoblastic cells (HOBs, Scien Cell Research Laboratories) was evaluated with a spectrophotometric MTT assay that measures cellular mitochondrial activity as well as with a differentiation assay that measures alkaline phosphatase (ALP) activity. The two treatment groups were ProRoot MTA mixed with distilled water (3:1 powder: liquid ratio) and ProRoot MTA mixed with HPL (Zen-Bio) (3:1 powder: liquid ratio). For the control groups, HOBs were incubated under identical conditions but without MTA. MTT and Alkaline Phosphatase assays were performed after 48 and 72 hours incubation in a serum supplement growth medium (MEM) at 37C. Statistical analysis: Data were analyzed by ANOVA; 0.05 was used as a significance level. Results: No statistically significant differences were found between the treatment and control groups in both MTT and ALP assays (P >0.05). Conclusion: Gray ProRoot MTA Root Canal Repair Material was biocompatible in direct incubations with human osteoblastic cells. Under the standard growth conditions tested here, addition of HPL did not significantly affect the cellular responses to the MTA. Further studies should be conducted to evaluate potential effects of MTA mixed with HPL under suboptimal cell growth conditions.

Tariq M. Aqili

BDS / Endodontic resident
King Abdulaziz Univeristy

I graduated 2010 from King Abdulaziz University, Jeddah, Saudi Arabia. I started in UB 2015. I am taking master degree and endo post-grad certificate as well. I have 2 amazing daughters. Regeneration is my best part in our field because of some reasons. First of all, it is dealing with human cells that has a unique mechanism. Secondly, it is still not fully understood. My hobbies are playing soccer, photoshop, and watching movie.

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Dinh Nguyen

DDS/volunteer reseaech assistant
Ho Chi Minh City University of Medicine and Pharmacy

I was graduated 2002. I started work in University at Buffalo this year as a volunteer research assistant. I am interesting in biological researches. I have 3 amazing kids. My best hobby is reading books and novel.

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Rosemary Dziak

PhD
University of Rochester

Education and Training:
Postdoctoral Fellow, Pharmacology, Northwestern U. Medical School (1975)
PhD, Radiation Biology, University of Rochester (1973)
MS, Radiation Biology, University of Rochester (1969)
BS, Biology, College Misericordia (1967)
Employment:
Co-Director, Institute for Research and Education on Women and Gender, University at Buffalo (2005-present)
Professor, Oral Biology, SUNY at Buffalo (1989-present)
Interim Chair, Oral Biology, SUNY at Buffalo (2002-2004)
Director-DDS/Ph.D. combined training Program Director, Oral Biology, SUNY at Buffalo (1996-2006)
Associate Chairman, Oral Biology, Oral Biology (1994-2000)
Acting Associate Chairperson, Oral Biology, SUNY at Buffalo (1987)
Awards and Honors:
Award for Mentoring Dental Student Research (2015)
Charles S. Lipani Award for Service to UB School of Dental Medicine (2014)
Promising Inventor Award (2002)
NIH Research Career Development Award (1985)
NIH Research Career Development Award (1984)
Award for Contribution to Dental Student Research (1984)
NIH Research Career Development Award (1983)
NIH Research Career Development Award (1982)
NIH Research Career Development Award (1981)
NIH Research Career Development Award (1980)

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PR49 - Biocompatibility of MTA Mixed with Human Platelet Lysate



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