The objective of this study was to evaluate if a porous collagen scaffold promotes the differentiation of human dental pulp stem cells (DPSC) into odontoblast-like cells to achieve guided tissue regeneration. DPSCs were cultured in standard or odontoblastic differentiation medium either in monolayer or in contact with a collagen scaffold. The adhesion of DPSCs was evaluated at 6 and 24 h. The cell proliferation was investigated by using (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) (MTT) assay, at 3 and 5 days. Cellular morphology was evaluated by optical microscopy at 3 and 7 days. Calcified tissue formation and alkaline phosphatase activity (ALP) were also evaluated after 2, 3 and 4 weeks. Dentin sialophosphoprotein (DSPP) and osteocalcin (OC) were measured by Western blot. Data were analyzed by one-way ANOVA. Posteriori comparisons were performed using Tukey’s test. P values were declared significant at ≤ 0.05. Results showed that DPSCs adhere and proliferate in contact with the collagen scaffold. Production of calcified tissue and ALP was significantly more important when DPSCs were cultured on/in the collagen scaffold. Protein analysis demonstrated high expression of DSPP and OC which confirmed the odontoblast-like cell phenotype. It was concluded that the porous collagen scaffold could offer a favorable microenvironment to promote DPSCs differentiation into odontoblast-like cells.
Dre Maryse Boisvert is currently a second year resident in endodontics post-graduated program at Faculty of dentistry of Laval University in Quebec city, Canada. She completed her DMD degree at Faculty of dentistry in Laval University in 2007 and worked as a general dentist in the Quebec city area for ten years. During her summer semesters, as an undergraduated student, she did research in the microbiological field of periodontology. Her interest for regenerative endodontics procedures arose during her career as a general dentist. She is now an active member of the « Groupe de Recherche en Écologie Buccale" of Laval University and conducting her master degree in fundamental science. She is currently working with dental pulp stem cells and studying their effects on collagen-based scaffold.
Mrs. Mabrouka Salem is a PhD student at Laval University. She got her engineering degree in biology from University in Tunisia then reached the CHUL-U. Laval research center to complete her MSc and PhD.
Dr. Hyunjin Park has trainings in cell biology (BSc), chemical engineering (MScA), bone regeneration (PhD) and wound healing/biocompatibility (Postdoc). She has expertise on cellular and molecular biology. She contributed to several students's trainings, basically during her post-doctoral formation period. Dr. Park published more than 10 papers in high impact per-review journals.
Endodontist and teacher
Dre Juliana Santos is an endodontist and an active member of the AAE. She obtained her DMD degree from Universidade Federal de Minas Gerais, Brasil, followed by a master and Ph.D. degree from Universidade Estadual de Campinas, Brasil. She obtained a master degree and certificate in
endodontics in 2013 at University of Louisville, Kentucky, USA. Dr Santos is an associate professor of endodontics and works as a full-time faculty
member at Laval University Dental School in Québec city, Canada.
Thursday, April 26
10:00 AM – 1:00 PM
Dr Mahmoud Rouabhia is a full-time professor at Faculty of Dentistry, Laval University, Quebec city, Canada. He is a senior scientist in the field of immunology, cell biology and tissue engineering. He did his Ph.D. in France followed by his post-doctoral training for four years in Canada. Dr Rouabhia's research interests include studying the interactions between human cells biomaterials and electrical stimulation for better wound healing. Dr Rouabhia have more than 130 peer-reviewed scientific publications. He also published more than 15 book chapters and reviewed articles and holds 2 patents. He is the editor or co-editor of two books in the field of tissue engineering and wound healing.