Poster Presentation

Poster Presentation

PR103 - Disruption of Enterococcus Faecalis Biofilm by a Novel Green Synthesized Graphene Oxide-Silver Nanocomposite

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

The ability of a novel, green synthesized graphene oxide-silver nanoparticle (GO-AgNp, Group 1) to disrupt a biofilm of Enterococcus faecalis and kill E.faecalis was studied, in comparison with 2% chlorhexidine gel (CHX, group 2), calcium hydroxide paste (CH, group 3) and a control (no treatment).  Mandibular premolars (n=40) with a 4 week biofilm of E.faecalis was randomly divided into 4 groups (n=10) based on the antimicrobial treatment. Control microbiological samples (S1) were obtained and medicaments were placed in the root canal. After 1 week, medicaments were removed and samples (S2) were obtained for CFU analysis. Biofilm architecture and dead cell % was analyzed using a confocal microscope (CLSM) within the root canal and dentinal tubules at 500 microns. The data (CFU values and dead cell %) were statistically analyzed [one way ANOVA and post hoc Tukey's tests (P=0.05)]. The biofilm architecture was disrupted in group 1, while group 2-4 showed an intact biofilm structure. Dead cell % varied by location; group 2 and group 1 showed significantly higher dead cell % within the root canal  and dentinal tubule respectively (P0.05). The graphene oxide-silver nano composite disrupted the biofilm, but CHX and CH could not. CHX was significantly better than GO-AgNP and CH in bacterial killing.

Prasanna Neelakantan

Clinical Assistant Professor
The University of Hong Kong

Endodontic specialty training from India and doctorate from ACTA/University of Amsterdam, the Netherlands. Clinical Assistant Professor and Assistant Dean for Research and Innovation at the Faculty of Dentistry, The University of Hong Kong. Scientific advisory board member of Journal of Endodontics and referee for International Endodontic Journal. 82 published papers till date with h index 18.

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