Poster Presentation

Poster Presentation

PR106 - Dimethyloxalylglycine-Embedded Poly(ε-caprolactone) Fiber Meshes Promote Odontoblastic Differentiation of Human Dental Pulp-Derived Cells

Thursday, April 26
10:00 AM - 1:00 PM
Room: Exhibit Hall

Objective: The effect of prolyl hydroxylase (PHD) inhibitors on pulp-dentin complex was investigated with in vivo transplantation model. The purpose of this study was to investigate the effect of DMOG-embedded poly(ε-caprolactone) fiber (PCLF/DMOG) on odontoblastic differentiation of human dental pulp-derived cells (hDPCs) by transplantation of the dentin slice model. Methods: hDPCs were seeded onto electrospun PCLF and PCLF/DMOG in dentin slices and then transplanted into nude mice. The surface topography was evaluated for both PCLFs, and DMOG release from the PCLF/DMOG was examined. The effects of the PCLF/DMOG were assessed by histology and RT-qPCR. Results: The PCLF/DMOG treated dentin slices showed higher cellularity with a palisading arrangement of hDPCs and organized collagen fibers. We found that the PCLF/DMOG significantly stimulated the expression of VEGFA, DSP, and BSP in the hDPCs (P < .05) and mVegfa, mPecam 1, and mNefl in the surrounding host cells (P < .05). Conclusions: These results show that PCLF/DMOG has potential in pulp-dentin complex regeneration by promoting odontoblastic differentiation of hDPCs and by enhancing host cell recruitment, angiogenesis, and neurogenesis, through the released DMOG-mediated cell responses.

YeonJee Yoo

Clinical Professor
Seoul National University

She graduated PhD course in endodontics, conservative dentistry in 2014. now working as a clinical professor. ongoing studies include regenerative endodontics, root canal instrumentation, intracanal medication and irrigation, endodontic microbiology, and endodontic surgery.1. We investigated the effect of DMOG-embedded poly(ε-caprolactone) fiber (PCLF/DMOG) on odontoblastic differentiation of human dental pulp-derived cells (hDPCs) through transplantation of dentin slice model to nude mice
2. Transplanted hDPCs seeded on plain PCLF and PCLF/DMOG were harvested and underwent immunohistologic and molecular analyses.
3. PCLF/DMOG treated dentin slices showed higher cellularity, with palisading arrangement of hDPCs and organized collagen fibers. RT-qPCR showed that DMOG/PCLF significantly stimulated the expression of VEGFA, DSP, and BSP in hDPCs (P < .05), and mVegfa, mPecam 1, and mNefl in surrounding host cells (P < .05).
4. Sustained release of DMOG from PCLF could promote odontoblastic differentiation of hDPCs, and enhance host cell recruitment, angiogenesis, and neurogenesis.

Presentation(s):

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Seung Ho Baek

Dimethyloxalylglycine-Embedded Poly(ε-caprolactone) Fiber Meshes Promote Odontoblastic Differentiation of Human Dental Pulp-derived Cells
Seoul National University

1. We investigated the effect of DMOG-embedded poly(ε-caprolactone) fiber (PCLF/DMOG) on odontoblastic differentiation of human dental pulp-derived cells (hDPCs) through transplantation of dentin slice model to nude mice
2. Transplanted hDPCs seeded on plain PCLF and PCLF/DMOG were harvested and underwent immunohistologic and molecular analyses.
3. PCLF/DMOG treated dentin slices showed higher cellularity, with palisading arrangement of hDPCs and organized collagen fibers. RT-qPCR showed that DMOG/PCLF significantly stimulated the expression of VEGFA, DSP, and BSP in hDPCs (P < .05), and mVegfa, mPecam 1, and mNefl in surrounding host cells (P < .05).
4. Sustained release of DMOG from PCLF could promote odontoblastic differentiation of hDPCs, and enhance host cell recruitment, angiogenesis, and neurogenesis.

Presentation(s):

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Kyung Mi Woo

professor
Seoul National University

1. We investigated the effect of DMOG-embedded poly(ε-caprolactone) fiber (PCLF/DMOG) on odontoblastic differentiation of human dental pulp-derived cells (hDPCs) through transplantation of dentin slice model to nude mice
2. Transplanted hDPCs seeded on plain PCLF and PCLF/DMOG were harvested and underwent immunohistologic and molecular analyses.
3. PCLF/DMOG treated dentin slices showed higher cellularity, with palisading arrangement of hDPCs and organized collagen fibers. RT-qPCR showed that DMOG/PCLF significantly stimulated the expression of VEGFA, DSP, and BSP in hDPCs (P < .05), and mVegfa, mPecam 1, and mNefl in surrounding host cells (P < .05).
4. Sustained release of DMOG from PCLF could promote odontoblastic differentiation of hDPCs, and enhance host cell recruitment, angiogenesis, and neurogenesis.

Presentation(s):

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WooCheol Lee

professor
Seoul National University

1. We investigated the effect of DMOG-embedded poly(ε-caprolactone) fiber (PCLF/DMOG) on odontoblastic differentiation of human dental pulp-derived cells (hDPCs) through transplantation of dentin slice model to nude mice
2. Transplanted hDPCs seeded on plain PCLF and PCLF/DMOG were harvested and underwent immunohistologic and molecular analyses.
3. PCLF/DMOG treated dentin slices showed higher cellularity, with palisading arrangement of hDPCs and organized collagen fibers. RT-qPCR showed that DMOG/PCLF significantly stimulated the expression of VEGFA, DSP, and BSP in hDPCs (P < .05), and mVegfa, mPecam 1, and mNefl in surrounding host cells (P < .05).
4. Sustained release of DMOG from PCLF could promote odontoblastic differentiation of hDPCs, and enhance host cell recruitment, angiogenesis, and neurogenesis.

Presentation(s):

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PR106 - Dimethyloxalylglycine-Embedded Poly(ε-caprolactone) Fiber Meshes Promote Odontoblastic Differentiation of Human Dental Pulp-Derived Cells



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