General autoimmunity

Poster

Coxsackievirus b3 Viral Protein 1 Possesses Multiple Immunodominant Epitopes That Induce Differential Antigen-specific, cd4 and cd8 T Cell Responses in A/j Mice

Thursday, June 15
5:45 PM - 7:00 PM

Coxsackievirus B3 (CVB) is commonly implicated in myocarditis that can lead to dilated cardiomyopathy possibly by triggering an autoimmune response, and there are no commercially available vaccines to prevent this infection in humans. In fact, by using major histocompatibility complex (MHC) class II dextramers for cardiac myosin, we recently demonstrated that A/J mice infected with CVB can show the generation of myosin-specific CD4 T cells that can transfer disease to naïve recipients. Thus, it is critical to design vaccines that are free of side effects like autoimmunity. We report here identification of viral protein1 (VP1) epitopes that permit us to track the generation of virus-specific T cell responses in infected animals. These include VP1 681-700, VP1 721-740 and VP1 771-790. While, all epitopes induce CD4 T cell responses as evaluated by proliferation assay and MHC dextramer staining, VP1 721-740 and VP1 771-790 could also induce CD8 T cell responses. Furthermore, by creating T cell hybridomas for VP1 771-790, we demonstrate that a proportion of splenocytes containing mononuclear cells express viral proteins in CVB infected mice. Together, the use of MHC dextramers in conjunction with T cell hybridomas may permit evaluation of antigen-specific T cell responses in infection and vaccine studies with CVB.

Rakesh H. Basavalingappa

University of Nebraska - Lincoln

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    Arunakumar Gangaplara

    University of Nebraska - Lincoln

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      Chandirasegaran Massilamany

      University of Nebraska - Lincoln

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        Bharathi Krishnan

        University of Nebraska - Lincoln

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          Ting Jia

          University of Nebraska - Lincoln

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            David Steffen

            University of Nebraska - Lincoln

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              Jay Reddy

              University of Nebraska - Lincoln

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                Coxsackievirus b3 Viral Protein 1 Possesses Multiple Immunodominant Epitopes That Induce Differential Antigen-specific, cd4 and cd8 T Cell Responses in A/j Mice



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