Immune monitoring

Poster

Production and Use of External Standards for Antigen-specific T-cell Assays

Friday, June 16
6:15 PM - 7:30 PM

Immunological assays for monitoring antigen-specific T-cell responses like Elispot, ICS, or MHC-multimer staining, are complex and prone to significant variation at all assay stages (e.g. sample acquisition, storage, shipment, processing, and analysis). However, these assays are generally poorly standardized. In particular, the lack of external assay standards reduces the comparability of results between runs and across institutions. A recently established technology provides a new type of reference T-cell sample based on T-cell-receptor-engineering. These T-cell engineered reference samples (TERS) can be adjusted with respect to the frequency of specific TCR-bearing T-cells in the sample, even permitting the use of high and low controls from the same source in parallel. They deliver stable signals over time and can be used in connection with stimulation based assays (Elispot, ICS) or MHC-multimer staining. The recent commercial availability of TERS kits with different TCR-specificities allows users to produce their own tailor-made batches of TERS and is likely to become a game changer in regards to the use external standards for the mentioned assay platforms. Here, we show our initial results produced with commercially available TERS kits, highlighting their usefulness for example in assay calibration and assay monitoring over time. The use of TERS facilitates the detection of even small variations in assay performance and may ultimately allow users to accept or reject assay runs based on an objective control. Our data showcases both the ease of use and the robustness of this approach on different platforms and highlights the advantages associated with proper assay standards.

Nicole Bidmon

Biontech, Mainz (Germany)

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    Pavlo Holenya

    JPT Peptide Technologies, Berlin (Germany)

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      Maren Eckey

      JPT Peptide Technologies, Berlin (Germany)

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        Aaron Castro

        Theracode JPT Inc. (US)

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          Florian Kern

          JPT Peptide Technologies, Berlin (Germany) /Brighton and Sussex Medical School (UK)

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            Ulf Reimer

            JPT Peptide Technologies, Berlin (Germany)

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              Holger Wenschuh

              CEO
              JPT Peptide Technologies, Berlin (Germany)

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                Sebastian Attig

                TRON Mainz (Germany)

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                  Tana Omokoko

                  Biontech, Mainz (Germany)

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                    Petra Simon

                    Biontech, Mainz (Germany)

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                      Richard Rae

                      TRON Mainz (Germany)

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                        Ugur Sahin

                        CEO
                        Biontech, Mainz (Germany)

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                          Sjoerd Van der Burg

                          Leiden University Medical Centre (Netherlands)

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                            Cecile Gouttefangeas

                            University of Tübingen (Germany)

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                              Marij Schoenemakers-Welters

                              Leiden University Medical Centre (Netherlands)

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                                Cedrik Britten

                                TRON Mainz (Germany)/GSK Stevenage (UK)* (*current affiliation)

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                                  Production and Use of External Standards for Antigen-specific T-cell Assays



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