Autoimmune rheumatologic diseases

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Ultrasensitive Assays to Measure Saliva and Plasma Cytokines in Primary Sjögren’s Syndrome and Sicca Control Donors - Association of Cytokines with Clinical Traits

Thursday, June 15
5:45 PM - 7:00 PM

Primary Sjögrens’ syndrome (pSS) is a chronic autoimmune disease characterized by inflammation and loss of secretory function of the exocrine glands.Both presence of Th17cells in salivary glands as well as elevated levels circulating IL-17 have been reported, indicating the involvement of this cell population in pSS.Although Tcells predominate in early lesions,B cells predominate later.Chronic Bcell activation plays an important role in the pathogenesis of pSS and leads to increased risk of lymphoma.Overexpression of BAFF on one hand and IL-21 on the other hand are thought to be critically involved in the enhanced plasmacell formation in pSS patients.
Therefore in this study we measured BAFF,IL-21 and IL-17A both in proximal (saliva) and peripheral (plasma) biofluids.Existing assays for these cytokines were not sensitive enough for saliva.We used novel ultrasensitive platforms to measure these cytokines in order to investigate the role of these biomarkers associated with these Bcell and Th17cell pathways in pSS and sicca patients.All cytokine assays were validated for sensitivity and specificity in their respective matrices.Saliva cytokine levels were normalized to flow rates.SalivaBAFF and plasmaIL-21 levels were significantly elevated in pSS vs. sicca patients. Plasma and saliva BAFF were significantly elevated in pSS patients who tested positive vs.negative for impaired salivary flow and lymphocytic infiltrates in salivary gland biopsies.PlasmaIL-17A was significantly higher in pSS patients positive versus negative for dry mouth and salivary gland lymphocytic infiltration.Cytokines in saliva and plasma were measurable at single femtogram concentrations offering hitherto unattainable detection sensitivities,particularly in saliva,enabling testing of patient stratification strategies in clinical trials. 

Tanushree Samanta

Senior Biologist
Eli Lilly & Co

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    Kathy Sivilis

    Oklahoma Medical Research Foundation

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      Kiely Grundahl

      Oklahoma Medical Research Foundation

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        Darise Farris

        Oklahoma Medical Research Foundation

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          Jennifer Kelly

          Oklahoma Medical Research Foundation

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            Ching-Yun Veavi Chang

            Research Scientist
            Eli Lilly & Co.

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              Sean Sissons

              Associate Consultant Biologist
              Eli Lilly & Co.

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                Josh Poorbaugh

                Consultant Biologist
                Eli Lilly & Co.

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                  Karen L. Cox

                  Senior Research Scientist
                  Eli Lilly & Co.

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                    Angus J. MacDonald

                    Principal Research Scientist
                    Eli Lilly & Co.

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                      Robert JAN.. Benschop

                      Senior Director
                      Eli Lilly & Co.

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