Allergy/asthma

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Immune Memory Is Established at the Incipient, Preclinical Stages of Food Allergy

Thursday, June 15
5:45 PM - 7:00 PM

Most patients with food allergy experience clinical symptoms at their first known exposure. A pre-requisite for the expression of such symptoms is the development of allergen-specific IgE that facilitates mast cell and basophil degranulation upon subsequent allergen exposure. Here, we searched for immunological events that might precede the generation of allergen-specific IgE. As these potential events are silent, we used a murine system. Mice were orally exposed only once to a food allergen (peanut or ovalbumin) with cholera toxin (CT), a classical Th2 adjuvant. Under these conditions, mice lacked allergen-specific germinal centres in the lamina propria (LP) of the small intestine and mesenteric lymph nodes (mLNs). They also had undetectable levels of allergen-specific IgE in serum and allergen-specific plasma cells in the bone marrow.  Accordingly, these mice did not experience anaphylaxis on systemic allergen challenge. However, we detected allergen-specific IgG1+ B cells in LP, spleen and mLNs by flow cytometry. In addition, stimulation of splenocytes with allergen in vitro induced CD4+ T-cell proliferation and Th2-associated cytokine secretion. When these mice were orally re-exposed to allergen without adjuvant, they developed allergen-specific IgE and underwent anaphylaxis on systemic challenge, even when allergen re-exposures were done 9 months after the initial, single exposure to allergen + CT. These findings indicate that long-lived allergen-specific memory is established after a single, clinically and humorally silent, immunogenic exposure to allergen, which is activated by inherently innocuous allergen exposures. These findings encourage further research to identify tools for the early prediction of food allergy.

Roopali Chaudhary

Post doctoral fellow
Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre (MIRC)

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    Rodrigo Jiménez-Saiz

    Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre (MIRC)

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      Joshua Koenig

      Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre (MIRC)

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        Alexandra Florescu

        Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre (MIRC)

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          Tina Walker-Fattouh

          Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre (MIRC)

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            Talveer S. Mandur

            Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre (MIRC)

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              Kelly Bruton

              Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre (MIRC)

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                Melissa E. Gordon

                Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre (MIRC)

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                  Yosef Ellenbogen

                  Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre (MIRC)

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                    Paul Spill

                    Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre (MIRC)

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                      Susan Waserman

                      Departments of Medicine and Pathology and Molecular Medicine, McMaster University

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                        Manel Jordana

                        Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre (MIRC)

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                          Immune Memory Is Established at the Incipient, Preclinical Stages of Food Allergy



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