Immune monitoring

Poster

High-dimensional Analysis of Human Regulatory T Cells Using Mass Cytometry

Friday, June 16
6:15 PM - 7:30 PM

Regulatory T cells (Tregs) are critical for maintenance of peripheral tolerance in a variety of tissues. Evidence that Tregs seem to be a phenotypically diverse population that varies between individuals and tissues suggests that there may be functionally-specialized subsets of Tregs which vary depending on location and/or disease state. We developed a new mass cytometry-based method to directly measure FOXP3 in combination with 20 other parameters to characterize the phenotype of human Tregs in different tissues, and to enable analysis of antigen-specific cells from whole blood. We first developed and characterized a protocol to directly detect FOXP3, the Treg lineage-defining transcription factor, via mass cytometry. We found this optimized protocol is more sensitive than commercially-available methods to detect FOXP3 by mass cytometry and is compatible with staining of whole blood, previously-frozen samples and other intracellular targets such as cytokines and other transcription factors. To ask how Treg populations differ depending on location and disease state, we stained mononuclear cells from adult peripheral blood, pediatric thymus and cord blood, as well as synovial fluid from pediatric subjects with juvenile idiopathic arthritis with our Treg-specific mass cytometry panel. The resulting data were analyzed using several bioinformatics approaches, including viSNE, which revealed diversity in the heterogeneity and phenotype of Tregs depending on their origin. The ability to stain FOXP3 effectively using mass cytometry will facilitate the further characterization of Tregs in health versus disease to understand how Tregs are functionally specialized in the context of different tissues and disease.

Nicholas Dawson

University of British Columbia

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    Avery Lam

    University of British Columbia

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      Romy Hoeppli

      University of British Columbia

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        Anne Pesenacker

        University of British Columbia

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          Raewyn Broady

          University of British Columbia

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            Megan Levings

            Professor
            University of British Columbia

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