Immuno-oncology

Poster

Radiation Combined with il-37 Enhances Antitumor Effects in du145 Prostate Cancer Cells

Wednesday, June 14
6:15 PM - 7:45 PM

Prostate cancer (PCa) is the one leading incidence of cancer in men in the USA, with the exception of skin cancer. Radiation therapy (RT) is a standard treatment for PCa, but there is toxicity that is caused by high radiation dosages, which causes side effects. PCa is categorized as a radio-resistant tumor. Thus, there is a need for a safe and efficient radiosensitizer that would decrease the radiation dosage. IL-37 is an IL-1 family member, which has been extensively studied in the immunological field. However, few studies have addressed the potential of IL-37 as a radiosensitizer. This study investigates whether or not IL-37 could sensitize PCa to radiation. Clonogenic survival assay, quick cell proliferation assay, immunohistochemistry (IHC), TUNEL staining, and caspase-3 activity assay kits were utilized to investigate the combined effect of IL-37 and RT (IL-37/RT) on cell proliferation, survival, and apoptosis of DU145, a PCa cell line. Further studies were performed by RT-PCR and IHC to evaluate the potential molecular mechanisms. IL-37/RT enhanced RT-induced inhibition of cell proliferation and apoptosis in DU145 cells. The expression of the anti-proliferative molecule, p27, increased and expression of the pro-proliferative molecule, cdk2, decreased which correlated with the decrease in cell survival and proliferation. The expression of pro-apoptotic molecules, Fas ligand and Bax, increased, which correlated with the increase of apoptosis.IL-37/RT enhances antitumor activity in PCa cells via modulation of anti- and pro-proliferative and pro-apoptotic molecules. These findings suggest that IL-37 has a potential as a radiosensitizer for PCa and warrants further investigation.

Vivi Ding

Des Moines University

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    Ziwen Zhu

    University of Missouri School of Medicine

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      Timothy Steele

      Chair and Professor of Microbiology and Immunology
      Des Moines University

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        Mark Wakefield

        Chair, Program Director, and Associate Professor - Division of Urology and Surgery
        University of Missouri School of Medicine

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          Yujiang Fang

          Academic Pathologist and Associate Professor of Pathology
          Des Moines University

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