Diabetes and other autoimmune endocrine diseases

Poster

Ultrasensitive Assays for Detection of Type 1 Diabetes (t1d) Autoimmune Reactivities

Thursday, June 15
5:45 PM - 7:00 PM

 In an ongoing study to assess the feasibility of developing multiplexed immunoassay panels for detection of organ-specific autoimmune biomarkers, MSD developed assays for the detection of T1D biomarkers. T1D affects approximately 0.3% of Americans and has a rapidly rising incidence. Autoimmune response markers of T1D can be detected prior to disease onset, serving as important tools for T1D research. MSD® MULTI-ARRAY technology was used to develop a first generation multiplexed assay to detect key T1D autoimmune response markers, namely, autoantibodies to glutamic acid decarboxylase (GADA), insulinoma 2 (IA2A), and insulin (IAA). Using assay proficiency evaluation samples from the Islet Autoantibody Standardization Program, these assays performed comparably to existing assays, with the advantages of low sample volume (( < 50 μL), high throughput, and no radioactivity. In the current study, next generation assays were developed using MSD’s ultrasensitive assay format to detect the T1D-relevant reactivities IAA, GADA, and IA2A. Preliminary data show assay sensitivities/specificities improve from 60%/100% to 90%/89% (IAA detection), 65%/100% to 70%/100% (GADA detection), and 35%/100% to 50%/100% (IA2A detection). Furthermore, a new ultrasensitive assay was developed for detection of another key biomarker, ZnT8 autoantibody, that exhibits preliminary sensitivity and specificity of 35% and 89%, respectively. The ultrasensitive MSD assay format significantly improves the ability to detect serum samples with multiple T1D reactivities.


The reported research was supported by the NIAID of the NIH under Award #1 U24 AI 118660. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.

Anu Mathew

Scientific Manager II
Meso Scale Diagnostics, LLC.

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    Mingyue Wang

    Scientist
    Meso Scale Diagnostics, LLC.

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      Simone Barbero

      Scientist
      Meso Scale Diagnostics, LLC.

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        Sunsanee Kanjananimmanont

        Research Associate
        Meso Scale Diagnostics, LLC.

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          Katarzyna Haynesworth

          Research Associate
          Meso Scale Diagnostics, LLC.

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            William E. Winter

            Professor, Pathology & Pediatrics
            University of Florida College of Medicine

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              David L. Pittman

              University of Florida College of Medicine

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                Jeff Debad

                Director
                Meso Scale Diagnostics, LLC.

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                  George Sigal

                  Director
                  Meso Scale Diagnostics, LLC.

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                    Jacob N. Wohlstadter

                    President and CEO
                    Meso Scale Diagnostics, LLC.

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