Cytokines/chemokines

Oral

Chemotaxis of Terminally Differentiated cd8+ Cells (temra) to the Sites of Sterile Infection: il8-receptors as an Answer?

Friday, June 16
6:15 PM - 7:30 PM

Terminally Differentiated Effector T cells (Temra) are defined as CD3+4/8+45RA+62L-CCR7- cells with poor proliferative capacity and contradictory functions, from exhausted cells to highly activated effector T cells (Teff). Recently, we revealed that enhanced levels of CD8+TEMRA are associated with delayed healing of bone fractures. Interestingly, the Temra accumulate in the fracture hematoma and release inflammatory mediators, which inhibit mesenchymal stem/progenitor cell differentiation into osteoblasts (Reinke et al. STM 2013). Similar evidence on the impact of Temra have been observed in other diseases. As Temra seem to be a heterogenous population and to better understand which subsets are involved, we started deeper analyses of Temra. After performing screening of >250 different surface molecules of 9 donors (CD-Screen), we could find out, that a subgroup of Temra specifically express IL8-Receptors and assumed it to be a chemotactical mechanism of Temra wandering to the sites of the sterile infection (e.g. bone fracture). The analysis of Temra by using a multiparameter panel via Masscytometry (CyTOF) showed simultaneous expression of some other interesting markers on this IL8R+ subpopulation of Temra (activation molecules, NK-cell markers etc.). Based on the identified phenotypic Temra subset, we have implemented the subset analysis into the clinical biomarker studies, comparing corresponding blood and hematoma samples. Moreover, sorting of the subsets and analysis of their functional and molecular properties as well as TCR repertoire are ongoing.

Iuliia Kotko

bcrt/imi, charite university medicine

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    Stephan Schlickeiser

    Institute for Medical Immunology, Charité

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      Simon Reinke

      bcrt, charite university medicine

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        Sven Geissler

        bcrt/jwi, charite university medicine

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          Désirée Kunkel

          BCRT, Charité University Medicine

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            Lisa-Marie Burkhardt

            IMI, Charité University Medicine

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              Mathias Streitz

              bcrt/imi, charite university medicine

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                Anke Jurisch

                bcrt/imi, charite university medicine

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                  Carolin Giannini

                  bcrt/imi, charite university medicine

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                    Hans-Dieter Volk

                    charite, inst.med.immunology and bcrt

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