Immunity & infection

Oral

In Situ Determination of Mif and cd74 in Skin Lesions of Lepromatous Leprosy Patients

Wednesday, June 14
6:15 PM - 7:45 PM

Leprosy is a chronic disease caused by Mycobacterium leprae that affects the skin and peripheral nerves; it may present as one of two distinct poles: the self-limiting tuberculoid leprosy and the highly infectious lepromatous leprosy (LL) characterized by M. leprae-specific absence of cellular immune response. The proinflammatory cytokine Macrophage Migration Inhibitory Factor (MIF) enhance the bactericide activities of macrophages after interaction with its receptor, CD74. MIF-mediated activation of macrophages is a key process for elimination of M. tuberculosis; however, its participation for the clearance of M. leprae is unclear. Particularly, it has not been studied in skin lesions of LL. The aim of this study was to evaluate MIF and CD74 in skin lesions of LL and compare it with healthy skin (HS) taken from subjects attending to dermatological consult. 37 biopsies of LL and 10 biopsies of healthy skin were analyzed by immunohistochemistry. Smears were observed in twelve 100X microscopic fields, in which percentage of stained cells and staining intensity were evaluated. Both variables were used to calculate a semiquantitative Score that ranged from 0 to 3+. Differences between groups were analyzed using Kruskar-Wallis test and Dunn-Bonferroni as post-hoc. We found no differences in MIF expression between the skins of LL and HS. We found CD74 Score statistically higher in LL skin than HS (p < 0.001); this was the result of a higher percentage of cells positive for CD74 (p < 0.001). As a conclusion, CD74-positive cells are intensely recruited to skins where lesions due to LL are observed

Marco Martinez

University of Guadalajara

Presentation(s):

    Send Email for Marco Martinez

    Anabell Alvarado-Navarro

    Universidad de Guadalajara

    Presentation(s):

      Send Email for Anabell Alvarado-Navarro

      Ana Laura Pereira Suárez

      Universidad de Guadalajara

      Presentation(s):

        Send Email for Ana Laura Pereira Suárez

        Mary Fafutis-Morris

        Universidad de Guadalajara

        Presentation(s):

          Send Email for Mary Fafutis-Morris


          Assets

          In Situ Determination of Mif and cd74 in Skin Lesions of Lepromatous Leprosy Patients



          Attendees who have favorited this

          Send Email for In Situ Determination of Mif and cd74 in Skin Lesions of Lepromatous Leprosy Patients