Immunity & infection

Poster

Contribution of Natural Killer T Cells to the Pathology Caused by the respiratory Syncytial Virus and the Metapneumovirus

Wednesday, June 14
6:15 PM - 7:45 PM

Natural killer T (NKT) cells are unconventional T cell lymphocytes. The NKT cell receptors recognize glycolipids bound to the MHC-I-like CD1d molecule. The synthetic α- Galactoceramides (α-Galcers) loaded onto CD1d has been shown to be a potent NKT cell activator, thereby these glycolipids have been explored as modulators of the immune response. Here, we describe the effect of α-Galcer on anti-viral immunity during the infection by the Human Respiratory Syncytial Virus (hRSV) and the Human Meptaneumovirus (hMPV). BALB/cJ mice were treated with α-Galcer and challenged either with hRSV or hMPV. α-Galcer treatment induced: 1) NKT proliferation in the lungs, spleen and LNs, 2) a significant reduction of neutrophil cells infiltration and loss weight amelioration in hMPV- but not in hRSV-infected mice and 3) a CD8+T cells recruitment to the lungs in the hRSV-infected mice. Preliminary data with BALBc CD1d-KO mice suggest that NKT cells can be important for the immune response against hMPV infection. To better understand as to how NKT cells modulate T cell immunity during in these infections, DCs were infected with hRSV or hMPV, pulsed with different concentrations of α-Galcer and co-cultured with NKT cells. While stimulation by hRSV-infected DCs led to reduced levels of IL2 secretion by NKT cells, hMPV-infected DCs promoted a significant IL2 secretion by NKT cells as compared to mock-treated DCs. Further, the observed increase of IL-2 by NKT cells stimulated with hMPV-infected DCs was α-Galcer dose dependent. Our data suggest that α-Galcer modulates NKT cell function, decreasing the exacerbated inflammation associated with hMPV but not for hRSV infection and modulating the IL-2 production in NKT cells co-cultured with hMPV-infected DCs.

Emma Rey-Jurado

Pontificia Universidad Catolica De Chile

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    Karen Bohmwald

    Pontificia Universidad Catolica de Chile

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      Nicolás Galvez

      Pontificia Universidad Católica de Chile

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        Daniela Becerra

        Pontificia Universidad Catolica de Chile

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          Leandro J. Carreño

          Universidad de Chile

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            Alexis M. M. Kalergis

            Titular professor
            Millennium Institute of Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile

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              Contribution of Natural Killer T Cells to the Pathology Caused by the respiratory Syncytial Virus and the Metapneumovirus



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