Immunity & infection

Poster

Recombinant Bcg Strain Induce Protective Humoral Immune Response Against the Human Respiratory Syncytial Virus (hrsv) and Human Metapneumovirus (hmpv)

Wednesday, June 14
6:15 PM - 7:45 PM


Millennium Institute of Immunology and Immunotherapy , Departamento de Genética Molecular y Microbiología , Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile , Santiago , Chile.


Human Respiratory Syncytial Virus (hRSV) and Human Metapneumovirus (hMPV) are two of the leading etiology agents of acute lower respiratory tract infections (ALRTIs) worldwide affecting young infants, elderly and immunocompromised patients. Currently, many approaches for the licensing of a safe and effective vaccine have been unsuccessfully tested. We have previously described that immunization with recombinant Mycobacterium bovis Bacillus Calmette-Guérin (BCG) strains expressing the HRSV Nucleoprotein (rBCG-N) and HMPV Phosphoprotein (rBCG-P) are able to induce protection through a T helper 1-induced cellular immunity. We show that upon viral challenge, immunization with these recombinant BCG strains promotes protective humoral immunity, characterized by the production of antibodies against several viral proteins. We detected a consistent isotype switching from IgG1 to IgG2a, which correlated with an increased viral clearance. Sera obtained from animals immunized with the BCG vaccines showed virus neutralizing capacity in vitro and protected naïve mice from pathology, by preventing viral replication and pulmonary disease. Finally, we found an increase in the plasma cells subset and in other B cells types in the immunized animals. These results support the notion that the use of recombinant BCG strains could be considered as potential vaccination approach against respiratory viruses, such as hRSV and hMPV.


Funding: Millennium Institute of Immunology and Immunotherapy.


CONICYT.

Jorge A. Soto

Millennium Institute of Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile
Pontifical Catholic University of Chile

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    Nicolás Galvez

    Pontificia Universidad Católica de Chile

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      Claudia RIvera

      Pontificia Universidad Católica de Chile

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        Christian Palavecino

        Pontificia Universidad Católica de Chile

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          Susan Bueno

          Millennium Institute of Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile

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            Alexis M. M. Kalergis

            Titular professor
            Millennium Institute of Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile

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              Recombinant Bcg Strain Induce Protective Humoral Immune Response Against the Human Respiratory Syncytial Virus (hrsv) and Human Metapneumovirus (hmpv)



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