Autoimmune neurologic diseases

No Preference

gpr15 Expression Is Increased in Smokers and Patients with Multiple Sclerosis and Is Associated with a ccr6+ T Helper Cell Phenotype

Thursday, June 15
5:45 PM - 7:00 PM

Background: Multiple sclerosis (MS) is an immune-mediated disease of multifactorial origin, targeting the central nervous system. Smoking is established as a risk factor associated with an increased risk of development and progression of MS. Circulating immune cells are believed to be involved in the pathogenesis of MS, but to what extent smoking affects circulating immune cells in MS is still unknown.


Objective and methods: With the present study we aimed to characterize differences in gene expression due to smoking in circulating immune cells of MS patients and healthy controls (HC). We used Affymetrix microarray and real-time qPCR gene expression analyses to identify genes associated with smoking in cohorts of MS patients and healthy controls. Flow cytometry analyses were used to further characterize immune cell phenotypes.


Results: Two genes, GPR15 and LRRN3, were upregulated in smokers in both MS patients and HC. GPR15 gene expression was also increased in MS patients compared with HC among non-smokers. GPR15 gene expression was further associated with increased frequencies of circulating CCD6+CXCR3- CD4+ T cells in smokers. In CSF of patients with relapsing-remitting MS, GPR15-expressing CD4+ T cells were enriched in CSF compared with blood.


Conclusion: Smokers and MS patients have increased GPR15 gene expression in blood which is associated with increased levels of Th17-like cells. In CSF from MS patients GPR15+ CD4+ T cells are enriched, suggesting a role of GPR15, an orphan chemokine receptor-like molecule, in pathogenic T cell recruitment in MS.

Cecilie Ammitzbøll

MD
Danish Multiple Sclerosis Center

Presentation(s):

    Send Email for Cecilie Ammitzbøll

    Helle B. Søndergaard

    MSc
    Danish Multiple Sclerosis Center

    Presentation(s):

      Send Email for Helle Søndergaard

      Lars Börnsen

      MD
      Danish Multiple Sclerosis Center

      Presentation(s):

        Send Email for Lars Börnsen

        Jeppe Romme Christensen

        MD
        Danish Multiple Sclerosis Center

        Presentation(s):

          Send Email for Jeppe Romme Christensen

          Annette B. Oturai

          Associate professor, MD
          Danish Multiple Sclerosis Center

          Presentation(s):

            Send Email for Annette Oturai

            Rikke Ratzer

            MD
            Danish Multiple Sclerosis Center

            Presentation(s):

              Send Email for Rikke Ratzer

              Marina R. von Essen

              Associate professor
              Danish Multiple Sclerosis Center

              Presentation(s):

                Send Email for Marina von Essen

                Finn Sellebjerg

                MD
                Danish Multiple Sclerosis Center

                Presentation(s):

                  Send Email for Finn Sellebjerg


                  Assets

                  gpr15 Expression Is Increased in Smokers and Patients with Multiple Sclerosis and Is Associated with a ccr6+ T Helper Cell Phenotype



                  Attendees who have favorited this

                  Send Email for gpr15 Expression Is Increased in Smokers and Patients with Multiple Sclerosis and Is Associated with a ccr6+ T Helper Cell Phenotype