Autoimmune neurologic diseases

Oral

Single-cell Phenotypic Analysis of Clonally Expanded cd8+ T Cells Reveals a Specific Clustering in Patients with Multiple Sclerosis

Friday, June 16
6:15 PM - 7:30 PM

Clonally expanded memory CD8+T cells are involved in multiple sclerosis (MS). We have shown that up to 87% of expanded CD8+T cells in brain-lesions of MS patients can be found in the cerebrospinal fluid (CSF) and 37% in the blood of the patients. We now aim to study CD8+T cells in the blood of MS patients at single-cell level to identify a molecular signature of pathogenic CD8+T cells and more precisely of the clonally expanded CD8+T cells.


To analyze single-cells from MS patients and controls, we used the C1 Single-Cell Auto-Prep System (Fluidigm) with subsequent microfluidic qPCR of 96 well-chosen genes and TCR Vβ-chain genes followed by sequencing. In parallel, we performed β-chainTCR immunosequencing on pooled memory CD8+T cells to analyze the repertoire and identify expanded clones.


We detected clear clustering and increased expression of more than 20 genes in MS patients compared to healthy volunteers (HV). Most of those genes are associated with CD8+T cell activation or MS. We also identified clonally expanded CD8+T for which we can compare the phenotype to the non-expanded CD8+T cells.


Our data are the first to describe a phenotypic analysis of CD8+T cells from MS patients at single-cell level and to link this phenotype to the TCR clonality. We detected in MS a gene expression profile which is clearly different to that of HVs. The gene expression differences and the clustering can serve as the basis to develop a biomarker to detect MS specific pathogenic cells in the peripheral compartments.

Emilie Dugast

Nantes University-INSERM U1064-Nantes CHU

Presentation(s):

    Send Email for Emilie Dugast

    Isabel Vogel

    INSERM U1064

    Presentation(s):

      Send Email for Isabel Vogel

      Alexandra Garcia

      Nantes University-INSERM U1064-Nantes CHU

      Presentation(s):

        Send Email for Alexandra Garcia

        Bryan Nicol

        Nantes University-INSERM U1064-Nantes CHU

        Presentation(s):

          Send Email for Bryan Nicol

          Jeremy Morille

          Nantes University-INSERM U1064-Nantes CHU

          Presentation(s):

            Send Email for Jeremy Morille

            Karin Tarte

            Inserm U917 - MICA - Faculté de médecine

            Presentation(s):

              Send Email for Karin Tarte

              Arnaud Nicot

              Nantes University-INSERM U1064-Nantes CHU

              Presentation(s):

                Send Email for Arnaud Nicot

                Laureline Berthelot

                Nantes University-INSERM U1064-Nantes CHU

                Presentation(s):

                  Send Email for Laureline Berthelot

                  Laure Michel

                  Nantes University-INSERM U1064-Nantes CHU

                  Presentation(s):

                    Send Email for Laure Michel

                    David-Axel Laplaud

                    Nantes University-INSERM U1064-Nantes CHU

                    Presentation(s):

                      Send Email for David-Axel Laplaud


                      Assets

                      Single-cell Phenotypic Analysis of Clonally Expanded cd8+ T Cells Reveals a Specific Clustering in Patients with Multiple Sclerosis



                      Attendees who have favorited this

                      Send Email for Single-cell Phenotypic Analysis of Clonally Expanded cd8+ T Cells Reveals a Specific Clustering in Patients with Multiple Sclerosis