Immuno-dermatology

Oral

Deciphering the Role of Dendritic Cells and Its Co-stimulatory Markers in the Immunopathogenesis of Pemphigus Vulgaris

Wednesday, June 14
6:15 PM - 7:45 PM

Pemphigus vulgaris (PV) is an autoimmune blistering disease of skin and mucous membranes characterized by auto-antibodies against Desmoglein3. The breach in immune tolerance, atypical T cell function and flawed antigen presentation are the predisposed factors in this disease. Dendritic cells (DCs) are crucial antigen presenting cells in maintaining tolerance and playing role in autoimmunity. The exact role of DC has not yet been explored in PV. In this study, DC frequency and phenotype was estimated using HLA-DR, Lin-, CD11c and CD123 markers by flowcytometry. A significant decrease in Myeloid DC (mDC) & Plasmacytoid DC (pDC) frequency in circulation of PV patients (n=20) vs. controls (n=20) was observed. Further, DC associated molecular markers were assessed in cultured DCs that were differentiated from monocytes and sorted by FACS Aria-III from the PBMCs of patients and controls. The mRNA levels of co-stimulatory/inhibitory molecules of cultured DCs and in circulation in PV patients were estimated. Expression levels of stimulatory molecules (CD40 & CD80) was found to be significantly increased, while a significant decline in inhibitory markers (PSGL1 & ILT3) was observed in sorted and cultured DCs from patients. Infiltrating Langerhans cells by IHC in perilesional tissue of PV patients suggests their involvement in this disease. The Lower frequency of mDC and pDC and increased expression of stimulatory molecules and decreased level of inhibitory molecules further strengthens the defect in functional status of DC. Thus, these co-stimulatory molecules might prove to be potential targets for novel therapeutics in PV.

Dayasagar Das

PhD Student
ALL INDIA INSTITUTE OF MEDICAL SCIENCES,NEW DELHI, INDIA

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    Parul Singh

    All India Institute of Medical Sciences , New Delhi, India

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      Sujay Khandpur

      Professor
      All India Institute of Medical Sciences , New Delhi, India

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        Sudheer KUMAR.. Arava

        Associate Professor
        All India Institute of Medical Sciences , New Delhi, India

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          Alpana Sharma

          Professor
          All India Institute of Medical Sciences , New Delhi, India

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