Autoimmune neurologic diseases

Oral

In-depth Immunophenotyping of Newly Diagnosed Relapsing Remitting Multiple Sclerosis (rrms) Patients and Patients Treated with Dimethyl Fumarate

Thursday, June 15
5:45 PM - 7:00 PM

There is limited information about the subpopulation dynamics in multiple sclerosis (MS) patients. Our aim is to assess the changes in peripheral immune responses of MS patients with and without treatments. We designed two multi-color panels (14 and 17 surface markers) to analyse cell surface expression of 41 various leukocyte subpopulations including B cells, monocytes, CD4+ and CD8+, NK cells and also dendritic cells by flow cytometry. To date, we have processed 12 healthy controls (HC), 53 relapsing remitting patients (32 treated with dimethyl fumarate (MS-DMF), and 21 newly diagnosed patients (MS-ND). In agreement with previous reports, MS-DMF had lower PBMC frequency than both HC and MS-ND (p values ≤0.003). T cell % was significantly different in MS-ND comparing to MS-DMF group (67.1% vs. 48.1%; p value 0.0001). Surprisingly, we observed lower CD8% in all MS patients comparing to HC (23.2% vs 34.1%; P value 0.0004). TCRγδ subpopulation was lower in the MS-DMF group vs. HC (1.28% vs. 3.2%; p value 0.0085). Also within CD4+ subpopulations, CD45RA- CD62L- was significantly lower in MS-DMF (6.6%) when compared to HC (17.0%) (P value 0.0001), Within B cell population, the B-cell subtype CD27+IgD- was 17.7% in HC, 7.9% in MS-DMF (P value 0.0017) and 16.1% in MS-ND (p value=NS versus HC or MS-DMF). The physiological importance of these observations are under investigation. Our data will shed new light on the mechanisms of inflammatory events in MS and also help to identify and predict patients' response to treatment.

Maryam Nakhaei-Nejad

Research Associate
University of Alberta

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    David Barilla

    University of Alberta

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      Gregg Blevins

      University of Alberta

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        Aaron Hirschfeld

        BD Biosciences

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          Fabrizio Giuliani

          Principal investigator
          University of Alberta

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            In-depth Immunophenotyping of Newly Diagnosed Relapsing Remitting Multiple Sclerosis (rrms) Patients and Patients Treated with Dimethyl Fumarate



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