General autoimmunity

Oral

cd19+cd3+ Cells: Heterogeneous Population That May Potentially Be Involved in Autoimmunity

Thursday, June 15
5:45 PM - 7:00 PM

In this study, we are interested in demonstrating the existence of CD19+CD3+ cells and whether they have a role in autoimmune diseases. We sought to evaluate whether CD3+CD19+ cells were differentially present in the peripheral blood of patients with autoimmune disease compared with healthy donors.


Peripheral blood was obtained from patients with confirmed diagnosis of autoimmune disease (Psoriasis; N=121, Multiple Sclerosis (MS); N=52, Sarcodiosis; N=66, Lupus (SLE); N=55, Inflammatory Bowel disease-IBD; N=33) who were untreated prior to sample acquisition or from healthy donors (N=53). Samples used for these studies were from NIH IRB-approved protocols. Phenotypic analysis of B and T cells was performed using multicolor FACS. Image-flow cytometry using AMNIS-image stream was used to analyze expression of markers at single cell level.


In comparison to the healthy controls, we observed a significantly higher frequency of CD3+CD19+ cells in MS, Sarcodiosis and Psoriasis but no differences in SLE or IBD. The CD3+CD19+ cells were found to be expressing CD20, HLA-DR, CD23, and CD27. CD86, Ig, CD38, CCR7.


In summary, we show that in autoimmune diseases the presence of a previously neglected T cell subset, CD3+CD19+ T cells. Further studies will be required to understand the developmental origin and evolutionary relevance of CD3+ CD19+ cells and whether they differ functionally from CD19- or recently discussed CD20+ T cells. Understanding the pathological relevance of this T cell subset in autoimmune disorders will likely broaden our understanding of human autoimmunity and may reveal novel therapeutic avenues.

Ankit Saxena

Research Fellow
NIH

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    Dagur Pradeep Kumar

    Staff Scientist
    National Institutes of Health

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      John J Philip McCoy Jr

      Core Director
      National Institutes of Health

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