Autoimmune rheumatologic diseases

Oral

Quantitative Profile of Innate and Adaptive Immune Response Abnormalities Related to Disease Activity in Patients with Systemic Lupus Erythematosus: Preliminary Results of a Prospective Cohort Study

Wednesday, June 14
6:15 PM - 7:45 PM

Introduction: Patients with systemic lupus erythematosus (SLE) show global immunologic abnormalities, some of them may be related to disease flare. The aim of this study was to correlate diverse innate and adaptive immunologic abnormalities with disease activity in a cohort of SLE patients. Methods. Fifty-two patients with SLE were prospectively followed-up during a median time of 6 (1-9) months. Clinical features were assessed at baseline, one and three months later. We assessed disease activity with the SELENA/SLEDAI scale. PBMCs were isolated by density gradient and the percentage and absolute numbers of CD4+, CD8+, NK cells, monocytes subsets, Th subsets, Tregs and B lymphocytes as well as TLR2 mean fluorescence intensity and percentage and absolute number of monocytes subsets expressing TLR2 were assessed by means of multiparametric flow cytometry.Results: At baseline, patients with constitutional symptoms had lower CD4+ cells, p=0.019, B lymphocytes, p=0.034 and higher percentage of NK cells, p=0.036. Subjects with hematologic activity had higher percentage of Th17 cells, p=0.006 and patients with SN involvement had higher Th1 percentage, p=0.06. Variables inversely correlated with severe flare at baseline and 3 months later were: percentage of CD4+, ρ -0.41, p=0.047, of NK ρ -0.52, p=0.028, of CD8+, ρ -0.56, p=0.015, of B lymphocytes ρ -0.55, p=0.021, and number of lymphocytes ρ -0.54, p=0.025 and percentage of TLR2 positive monocytes ρ -0.36, p=0.045.Conclusions: Low CD4+, CD8+, NK and B cells at baseline and lower TLR2 positive monocytes in the following 3 months correlated with severe SLE flare according to the SELENA/SLEDAI scale.



Diana Gómez-Martín

Research Scientist
Instituto Nacional de Ciencias Médicas y Nutrición, Salvador Zubirán

Presentation(s):

    Send Email for Diana Gómez-Martín

    Jiram Torres-Ruíz

    Instituto Nacional de Ciencias Médicas y Nutrición, Salvador Zubirán

    Presentation(s):

      Send Email for Jiram Torres-Ruíz

      Javier Merayo-Chalico

      Instituto Nacional de Ciencias Médicas y Nutrición, Salvador Zubirán

      Presentation(s):

        Send Email for Javier Merayo-Chalico

        Roberto Reyna-de la Garza

        Instituto Nacional de Ciencias Médicas y Nutrición, Salvador Zubirán

        Presentation(s):

          Send Email for Roberto Reyna-de la Garza

          Sandra Morales-Padilla

          Instituto Nacional de Ciencias Médicas y Nutrición, Salvador Zubirán

          Presentation(s):

            Send Email for Sandra Morales-Padilla

            Ricardo Vázquez-Rodríguez

            Instituto Nacional de Ciencias Médicas y Nutrición, Salvador Zubirán

            Presentation(s):

              Send Email for Ricardo Vázquez-Rodríguez

              Jorge Alcocer-Varela

              Instituto Nacional de Ciencias Médicas y Nutrición, Salvador Zubirán

              Presentation(s):

                Send Email for Jorge Alcocer-Varela


                Assets

                Quantitative Profile of Innate and Adaptive Immune Response Abnormalities Related to Disease Activity in Patients with Systemic Lupus Erythematosus: Preliminary Results of a Prospective Cohort Study



                Attendees who have favorited this

                Send Email for Quantitative Profile of Innate and Adaptive Immune Response Abnormalities Related to Disease Activity in Patients with Systemic Lupus Erythematosus: Preliminary Results of a Prospective Cohort Study