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Increased T-cell Activation and Differentiation in Patients with Alcohol Use Disorder

Wednesday, June 14
6:15 PM - 7:45 PM

Chronic alcohol consumption promotes the activation and differentiation of T-cells. These effects have been associated with increased apoptosis, reduced ability to recognize new antigens, and risk of autoimmunity. We aimed to analyze the profiles of CD4 and CD8 T-cell activation and differentiation in patients with alcohol use disorder (AUD)


Patients and Methods: cross-sectional study in patients seeking detoxification. Blood samples for immunophenotyping (FACSCalibur, BD Biosciences) were obtained the first day of admission. CD4 and CD8 T-cells subpopulations were studied using combinations of monoclonal antibodies for activation (anti-HLADR V500 and anti-CD38 APC) and differentiation (anti-CD45RA PECy7 and anti-CCR7 PE). Patients under immunosuppressive therapy and/or HIV infection were excluded. We analyzed 50 healthy blood donors as controls.


Results: 79 patients were included (81% M); median age was 50 years [IQR:45-56 yrs] and, alcohol consumption was 150g/d [IQR:100-200]. With respect to controls, patients had higher expression of CD4+CD38-HLADR+ and CD4+CD38+HLADR+ activation phenotypes (p < 0.05) and higher expression of all CD8+ activation profiles (p < 0.001). Regarding T-cell differentiation, patients showed lower proportions of naïve CD4+ T-cells (CCR7+CD45RA+) (32±25% vs. 41± 14%, p) and naïve CD8+ T-cells (22±15% vs. 39±17%, p < 0.001). The percentage of Central Memory (CCR7+CD45RA-) and Effector Memory (CCR7-CD45RA-) of CD4+ and CD8+ T-cells were increased in patients than in controls (p < 0.05).
Conclusions: an increased expression of T-cell activation and differentiation is observed in patients with alcohol use disorder. These findings could help to identify mechanisms of both immunodeficiency and/or inflammation in this population.

PAOLA ZULUAGA

HOSPITAL GERMANS TRIAS I PUJOL, UNIVERSITAT AUTONOMA DE BARCELONA

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    Arantza Sanvisens

    HOSPITAL GERMANS TRIAS I PUJOL, UNIVERSITAT AUTONOMA DE BARCELONA

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      Daniel Fuster

      HOSPITAL GERMANS TRIAS I PUJOL, UNIVERSITAT AUTONOMA DE BARCELONA

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        Aina Teniente

        HOSPITAL GERMANS TRIAS I PUJOL, UNIVERSITAT AUTONOMA DE BARCELONA

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          Eva Martínez-Cáceres

          HOSPITAL GERMANS TRIAS I PUJOL, UNIVERSITAT AUTONOMA DE BARCELONA

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            Jordi Tor

            HOSPITAL GERMANS TRIAS I PUJOL, UNIVERSITAT AUTONOMA DE BARCELONA

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              Roberto Muga

              HOSPITAL GERMANS TRIAS I PUJOL, UNIVERSITAT AUTONOMA DE BARCELONA

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