Immuno-oncology

Poster

Wnt Signaling Inhibitor xav939 Potentiates Cytotoxic Activity of Prostate Cancer Patient′s Lymphocytes in Vitro

Wednesday, June 14
6:15 PM - 7:45 PM

Inhibitors of Wnt signaling are contemporarily aimed for several clinical studies as adjuvant anti-cancer agents. As such these inhibitors might also be considered as promising molecules that could affect cytotoxic impact of prostate cancer (PCa) patient′s lymphocytes on PCa cells. To test this hypothesis, we generated stably transfected LNCaP PCa cell line with a red fluorescent protein TagFP635. Lymphocytes from patients with biochemically recurrent prostate cancer were cultured for 2 days in the presence of 20 U/ml IL-2 with or without Wnt signaling inhibitor XAV939. The TagFP635-LNCaP cells were then co-cultured with lymphocytes from patients with PCa at ratio 1: 4 for 3 days. The growth of LNCaP cells was then evaluated by fluorescence microscopy using image analysis. We found that PCa patient′s lymphocytes slowed down growth of TagFP635-LNCaP cells by 35% compared to untreated tumor cells control. However, when the PCa patient′s lymphocytes were pre-treated with 5μM Wnt signaling inhibitor XAV939 the growth of TagFP635-LNCaP cells was abrogated. No such changes were observed when TagFP635-LNCaP cells were co-cultured with XAV939 alone. Our data indicate that blocking Wnt signaling by XAV939 represents a potential way how to improve the patient`s lymphocytes cytotoxic impact on PCa cells.

Dmitry Stakheev

Department of Immunology, University Hospital in Motol, Prague

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    Zuzana Strizova

    MD
    Department of Immunology, University Hospital in Motol, Prague

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      Pavla Taborska

      Department of Immunology, University Hospital in Motol, Prague

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        Petra Vrabcova

        Department of Immunology, University Hospital in Motol, Prague

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          Michal Podrazil

          MD
          Department of Immunology, University Hospital in Motol, Prague

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            Jirina Bartunkova

            Department of Immunology, University Hospital in Motol, Prague

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              Daniel Smrz

              Department of Immunology, University Hospital in Motol, Prague

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                Wnt Signaling Inhibitor xav939 Potentiates Cytotoxic Activity of Prostate Cancer Patient′s Lymphocytes in Vitro



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