Therapeutics/pharmacology

Oral

Nanodiamonds as Novel Therapeutics to Treat Intracellular Bacterial Infections in Human Bladder Cells

Wednesday, June 14
6:15 PM - 7:45 PM

Recurrent urinary tract infections (UTIs) causing Escherichia coli (E. coli) pose a significant health threat by evading the immune system via internalization into host cells. In absence of vaccines, antibiotics are the main line of treatment for UTIs, but their overuse contributes towards bacterial resistance, resulting in worldwide public health concern. Thus, it is critical to develop novel therapeutics for UTIs. Nanodiamonds (NDs) are advantageous over conventional nanomaterials as they can be functionalized to adsorb therapeutics and develop novel, slow drug-releasing, targeted delivery systems. Safety studies demonstrate that NDs display minimal toxicity in variety of cells compared to carbon nanotubes. The objective of the current study was to investigate the ability of internalized NDs to kill E. coli that invade bladder cells.


We utilized an established in vitro bacterial invasion model, comprising of human bladder T24 epithelial cells infected with invasive E. coli. Acid-treated 6 and 25 nm NDs were tested for their capacity to kill bacteria by treating infected bladder cells with NDs for 24 hours. We found that 6 nm NDs were able to kill E. coli internalized in T24 cells more effectively than 25 nm NDs in a dose dependent manner (46.1% vs 81.1% at 100 μg/mL, P). We further confirmed actin-dependent internalization of 6 nm NDs by using cytochalasin D and determined that we could prevent the efficient killing of intracellular bacteria by inhibiting this internalization of NDs.These findings provide a foundation for establishing NDs as novel therapeutics to kill internalized bacteria causing recurrent UTIs.

Janaki Iyer

Post doctoral fellow
Oklahoma State University Center for Health Sciences

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    Alexia Dickey

    Oklahoma State University-Center for Health Science

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      Anil Kaul

      Oklahoma State University-Center for Health Sciences

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        Parvaneh Rouhani

        Oklahoma State University

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          Nirmal Govindaraju

          Oklahoma State University

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            Raj Singh

            Oklahoma State University

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              Rashmi Kaul

              Oklahoma State University

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