Immunodeficiency: primary or acquired

Oral

dock8 Deficient Germinal Center B Cells Rescued by a vav-bcl2 Transgene Undergo Affinity Maturation

Friday, June 16
6:15 PM - 7:30 PM

Analysis of the GC B cells in mice receiving Fas deficient wild type or DOCK8pri/pri SWHEL B cells showed similar results to Fas sufficient cells with a loss of DOCK8pri/pri GC B cells at day 9. By contrast, DOCK8pri/pri GC B cells carrying the VavBcl2 transgene were present at both day 9 and 15 after immunization, although there remained a significant decrease in GC B cells compared to wild-type. Sequencing revealed an overall acquisition of the Y53D mutation (indicating affinity maturation) in 34% of 126 DOCK8pri/pri VavBcl2 transgenic SWHEL GC B cells sequenced, and in 45% of 114 wild-type cells. This indicates that when the VavBcl2 transgene allows for GC B cell survival, mutations in DOCK8 do not prevent affinity maturation.

Katrina L. Randall

1. John Curtin School of Medical Research, Australian National University; 2. ANU Medical School, Australian National University

Presentation(s):

    Send Email for Katrina Randall

    Andrew F. Ziolkowski

    1. John Curtin School of Medical Research, Australian National University

    Presentation(s):

      Send Email for Andrew F. Ziolkowski

      Zahra Sabouri

      1. John Curtin School of Medical Research, Australian National University

      Presentation(s):

        Send Email for Zahra Sabouri

        Hsei Di Law

        1. John Curtin School of Medical Research, Australian National University

        Presentation(s):

          Send Email for Hsei Di Law

          Christopher C. Goodnow

          1. John Curtin School of Medical Research, Australian National University; 3. Garvan Institute of Medical Research

          Presentation(s):

            Send Email for Christopher C. Goodnow


            Assets

            dock8 Deficient Germinal Center B Cells Rescued by a vav-bcl2 Transgene Undergo Affinity Maturation



            Attendees who have favorited this

            Send Email for dock8 Deficient Germinal Center B Cells Rescued by a vav-bcl2 Transgene Undergo Affinity Maturation