Autoimmune rheumatologic diseases

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Plasma Mitochondrial Dna Increases and Levels of il-7, mcp-1 and Pdgf decrease in Ra Patients Treated with Biological Agents for >12 Months

Thursday, June 15
5:45 PM - 7:00 PM

Mitochondrial DNA (mtDNA), released upon tissue damage is a danger signal and a potent inflammatory trigger. The abundance of mtDNA is correlated with aging, frailty, cardiovascular diseases, diabetes, SIRS, and suggested as a cancer biomarker. We proposed to study its levels by quantitative real-time PCR in plasma from 19 rheumatoid arthritis (RA) patients before and at least 12 months after starting biological therapy (11 anti-TNFα, 5 CTLA-Ig and 3 anti-IL6R); clinical status was assessed by DAS28, ESR and CRP, and concentration of 40 cytokines was measured by Quantibody Human Inflammatory Array 3 (RayBiotech,Inc. Norcross, GA). Plasma mtDNA was lower (p < 0.05) in RA patients before biologicals compared with 20 age-matched healthy controls (mean 63 and 62 yrs) but it increased after therapy by 1log10on average (p < 0.01). We found no correlation between mtDNA levels and disease activity at baseline and follow-up; changes observed were unrelated to plasma values of cytokines studied, including TNFα and IL6 or IL6R. 33/40 cytokines decreased after biological therapy, with five (including TNFα and TNFRII) showing a significant reduction (p < 0.001). The others were MCP-1/CCL2, IL7 and PDGF-BB, produced by stromal cells, monocytes and synoviocytes. A correlation of these molecules with TNFα levels was observed at baseline but not after therapy. These growth factors may be markers of synovial hyperplasia and activity in RA, contributing to inflammatory damage, whereas the amount of plasma mtDNA seems not related to inflammation or disease activity in RA, as also found in SLE.

Roberto Paganelli

M.D. - Full Professor of Medicine
University G. D'Annunzio

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    Marika D'Urbano

    Technician
    Clinical chemistry Laboratory, ASL

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      Eleonora Celletti

      Clinical assistant, M.D.
      SS. Annunziata Hospital, Rheumatology outpatient clinic

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        Myriam Di Penta

        Fellow in training, Medicine
        SS. Annunziata Hospital, ASL

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          Milena Nasi

          Research Assistant
          2Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia

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            Marcello Pinti

            Professor
            Department of Life Sciences, University of Modena and Reggio Emilia

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              Andrea Cossarizza

              Full Professor of Immunology, General Pathology
              4Department of Medical and Surgical Sciences of Children and Adults, University of Modena and Reggio Emilia

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                Plasma Mitochondrial Dna Increases and Levels of il-7, mcp-1 and Pdgf decrease in Ra Patients Treated with Biological Agents for >12 Months



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