Autoimmune rheumatologic diseases
Introduction: Rheumatoid Arthritis (RA) is a chronic inflammatory disease characterized by pain, swelling and progressive destruction of multiple joints, affecting approximately 1% of the human population. The complement proteins have been known to play complex roles in the pathogenesis of RA.
Objectives: Studies on animal models suggest disease modulating activity of Leukocyte CR1 (L-CD35) and CR2 (L-CD21) in autoimmune disorders. Therefore, we aimed at elucidating a case control study to explore the role of L-CR1 and L-CR2 in RA patients.
Methodology: The L-CR1 and L- CR2 expression in 50 healthy controls and 50 RA patients on different leukocyte subpopulations was observed by Flow cytometry and expression at mRNA level was monitored by using RT- PCR. The clinical parameters Circulating Immune Complexes (CIC), C3, C3d and Disease activity scores (DAS28) were determined and correlated with L-CR1 and L-CR2 expression in patients.
Results: The L-CR1 and L-CR2 transcripts declined significantly in patients. A significant negative correlation of CR1 and significant positive correlation of CR2 transcript were observed with C3d and CIC in patients. C3 was correlated positively with CR2 transcript in patients. CR1 and CR2 transcript were negatively correlated with Disease activity.
Conclusion: Decline in the L-CR1 level suggests failure in protective role of L-CR1 against complement mediated damage in RA patients. Follow-up study confirming L-CR1 and L-CR2 role as pivotal biomarker is warranted. In essence, our findings suggest a close relationship of CR1 and CR2 with the pathophysiology and disease activity of RA and their importance as putative disease marker.
Post doctoral fellow
Indian Institute of Technology- Delhi
All India Institute of Medical Sciences, New Delhi, INDIA
Sr. Consultant & HOD – Biochemistry Director, Department of Lab Medicine
Nayati Multi Super Specialty Hospital, NH- 2, Mathura- 281003, U.P. , INDIA