Immunity & infection

Oral

Characterization of the Human B Cell Response in Dengue Infected Children from India: The Massive Expansion of Plasmabalsts parallels with the Timing of the Onset of Severe Disease

Wednesday, June 14
6:15 PM - 7:45 PM

Epidemiological studies suggest that India has the largest number of dengue cases world-wide. B cell responses are important in dengue because they are implicated in both protection and immunopathology. However, plasmablasts, the major antibody producing B cells remain poorly characterized in dengue and virtually no information is available on human plasmablast responses in the patients from India. Here we provide a first comprehensive description of plasmablasts in dengue patients from India. Samples from a total of 108 children confirmed as dengue by dengue specific NS1, IgM and/or PCR assays were analysed by multicolour flow cytometry using CD19, CD20 CD38, CD27 surface markers; proliferation characteristics using intracellular Ki67 staining, and the dengue specific antibody response using functional ELISPOT assays. Our studies show that plasmablasts expand massively in these dengue patients which was associated with loss of surface IgD, down regulation of CD20, and expression of Ki67. A vast majority of these massively expanding plasmablasts secreted dengue specific IgG isotype antibody. In some patients plasmablast frequencies reached as high as 80% of the B cells, translating to more than a million plasmablasts/ml of blood. Kinetic analysis of the plasmabalsts and dengue disease severity was also performed by stratifying the patients based on the day of symptoms revealed that the timing of the peak of this massive plasmablast expansion parallels with the time when a majority of the patients present with severe dengue disease. These studies open novel avenues to understand the role of plasmablasts in human dengue

Kaustuv Nayak

International Centre for Genetic Engineering and Biotechnology

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    Mohit Singla

    All India Institute of Medical Sciences

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      Sivaram Gunisetty

      Emory Vaccine Center, Emory University

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        Elluri Seetharami Reddy

        International Center for Genetic Engineering and Biotechnology

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          Harekrushna Panda

          International Center for Genetic Engineering and Biotechnology

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            Anil Verma

            All India Institute of Medical Sciences

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              Charu Aggarwal

              International Center for Genetic Engineering and Biotechnology

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                Guruprasad Medigeshi

                Translational Health Science and Technology Institute

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                  Rakesh Lodha

                  All India Institute of Medical Sciences

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                    Sushil Kabra

                    All India Institute of Medical Sciences

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                      Rafi Ahmed

                      Emory Vaccine Center, Emory University

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                        Murali-Krishna Kaja

                        Emory Vaccine Center, Emory University

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                          Anmol Chandele

                          International Center for Genetic Engineering and Biotechnology

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