Inflammatory bowel diseases

Poster

Therapeutic Potential of Recombinant Wuchereria Bancrofti l2 (rwbl2) in the Treatment of Dss-induced Chronic Ulcerative Colitis

Wednesday, June 14
6:15 PM - 7:45 PM

Current research advances for therapeutic interventions against inflammatory diseases including ulcerative colitis shows the use of helminths and their molecules. Hence, in present study, we investigated the anti-inflammatory and therapeutic effect of Wuchereria bancrofti L2 against dextran sulfate sodium (DSS)-induced chronic colitis in BALB/c mice.


Mice were administered with four cycles of DSS (4%) with one week interval after each cycle for the induction of chronic colitis. Mice were treated intraperitoneally with four consecutive doses of rWbL2 (25 μg in 200 μl PBS) starting from the 21st day of colitis induction. The body weight and disease activity index of mice were monitored daily. At the end of the experiment, colons were harvested for macroscopic and microscopic examination and also for the assessment of myeloperoxidase activity. The relative mRNA expression of the cytokines in the splenocytes was analyzed by real-time polymerase chain reaction.


The mice treated with rWbL2 ameliorated the disease severity measured in terms of disease parameters viz., disease activity index (p≤0.05), colon length (p≤0.005) and degree of mucosal edema (p≤0.005). Histopathological analysis revealed significantly improved macroscopic and microscopic and decreased myeloperoxidase activity, which correlated with the significant (p


In conclusion, above findings provide valid proof for exploring rWbL2 as a therapeutic candidate against ulcerative colitis.

Namdev SHIVAJI.. Togre

Ph. D. student
Mahatma Gandhi Institute of Medical Sciences

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    Priyanka SUDAM.. Bhoj

    Ph.D. student
    Mahatma Gandhi Institute of Medical Sciences

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      Vishal Khatri

      Post-doctoral Research Associate
      university of illinois

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        Aditya Tarnekar

        Professor
        Mahatma Gandhi Institute of Medical Sciences

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          Kaylan Goswami

          Professor
          Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences

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            MVR Reddy

            Director, Professor & Head
            Mahatma Gandhi Institute of Medical Sciences

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