Lymphatic filariasis is a major neglected disease of the tropics, caused by Brugia malayi and Wuchereria bancrofti parasites. These parasites are inhabitant of lymphatic vessels and ultimately choke the lymphatic circulation eventually leading to chronic lymphedema. Stagnation of lymph fluid nourishes the growth of bacteria and fungi predominantly in the dermal cells of lower limbs. Our present study aimed at evaluating the bacterial killing, free radical scavenging and cytokine inhibiting potential of thymol derived from Trachyspermum ammi. Thymol shows a clear zone of inhibition in paper disc and agar diffusion methods, indicating significant anti-bacterial activity against Bacillus cereus and Staphylococcus epidermis associated with filarial lymphedema. Further, thymol scavenges the DPPH (diphenyl-picryl-hydrazyl-hydrate) and Hydrogen peroxide (H2O2) free radicals at a dose dependent manner, indicating its inherent anti-oxidant property. In-vitro lymphangiogenic activity of thymol was evaluated by 2D matrigel and Nitric Oxide (NO) production in human endothelial cells in response to TNF-α (10ng/ml). These studies showed that thymol favours the formation of the tubular network and interestingly the NO production is elevated with increasing concentrations of thymol. Bacterial Infection Assay and Viable cell count analysis were carried out to evaluate the internalization of bacteria into the host cells and the viability of host cells after infection respectively. Real Time-PCR analysis and Confocal Imaging studies are under progress. Taken together, thymol efficiently kills bacteria, scavenges free radicals and induces lymphangiogenesis. Thus thymol derived from Trachyspermum ammi can be a novel therapeutic agent in the lymphatic filarial treatment.