Inflammatory bowel diseases

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A Novel cd177+ Population of foxp3+ Regulatory T Cells in the Intestinal Mucosa Is Decreased in Inflammatory Bowel Disease

Wednesday, June 14
6:15 PM - 7:45 PM

Background: CD177, the receptor for platelet endothelial cell adhesion molecule (PECAM-1, CD31), is expressed on neutrophils, but is not known to be on lymphocytes. We found CD177 mRNA selectively expressed by CD25+, CD127- regulatory T cells (Tregs) from human colon lamina propria lymphocytes (LPL).ÂÂ


Methods:  LPL from colonoscopic biopsies of 24 patient with or 9 without inflammatory bowel disease (IBD) were evaluated by flow cytometry. A pilot comparison of the whole genome mRNA expression of CD177+ and CD177- Tregs from the surgically resected colon of a single ulcerative colitis (UC) patient was performed.ÂÂ


Results: High levels of surface CD177 was expressed by up to 60% (mean 18%) of FOXP3+, Helios+, but not Helios-, LPL Tregs. The fraction of FOXP3+, Helios+ LPL Tregs expressing CD177 was smaller in LPL from patients with than without IBD. However, because there were more Tregs overall in IBD LPL, the total number of CD177+ Tregs was no lower in IBD biopsies. CD177+ Tregs had a gene expression profile similar to that of CD177- Tregs from the colon of a UC patient, except for a subset of genes up-regulated in the CD177+ population, including chemokines (specifically CXCR2 ligands), cytokines, cell surface receptors, and matrix proteins, suggesting a unique function for this population in mucosal immunity.ÂÂ


Conclusions: CD177+ Tregs represent a novel subpopulation of FOXP3+, Helios+ Tregs in the intestinal mucosa that is relatively under-represented in IBD, and may have a novel gene expression profile. ÂÂ

James DANIEL.. Lord

Research Assistant Member
Benaroya Research Institute, Translational Research Program

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    Donna Shows

    Benaroya Research Institute, Translational Research Program

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