Immunodeficiency: primary or acquired

Oral Abstract Sessions

Molecular and Cellular Mechanisms for B Cell Defects in Elderly and Obese Subjects

Thursday, June 15
3:13 PM - 3:26 PM

B cell function is decreased with age and obesity and associated with increased chronic low-grade systemic and metabolic inflammation. We have previously shown that obesity decreases the influenza vaccine response in young and elderly individuals and this is associated with a decrease in switched memory (swIg) B cells (CD19+CD27+IgD-) and an increase in late exhausted memory (LM) B cells (CD19+CD27-IgD-). We show here that elderly have more inflammatory cytokines (e.g.TNFa, IL-6) in their LM B cells and B, T, and monocytes from the obese subjects have significantly elevated IL-6, IL-17a, and TNF-a respectively as compared with their lean controls. Molecular markers of class switch recombination [activation-induced cytidine deaminase (AID), the transcription factor E47, and IgG in culture supernatants] are decreased in obese young and elderly as compared with their lean age-matched controls. To query possible mechanisms for these differences we have measured conditions and effects of visceral adipose tissue (VAT) on B cell phenotype and function. We found significantly more LM B cells in the VAT than in the blood. Moreover, adipocytes make pro-inflammatory chemokines (e.g. CXCL10, IL-8, CCL2, and CCL5) which could recruit B cells into the VAT and for which B cells have chemokine receptors. B cells from the VAT also express more pro-adipogenic markers (e.g. more Hormone-sensitive lipase and PPAR gamma and less SOD1). We have also shown that B cells from human blood co-cultured with adipocytes induce more LM B cells. These results show a direct effect of adipocytes on pro-inflammatory B cells

Bonnie B. Blomberg

Prof of Microbiology and Immunology
University of Miami Miller School of Medicine

Presentation(s):

Send Email for Bonnie Blomberg

Alain Diaz

U Miami Miller School of Medicine

Presentation(s):

Send Email for Alain Diaz

Maria Romero

U Miami Miller School of Medicine

Presentation(s):

Send Email for Maria Romero

Thomas Vazquez

U Miami Miller School of Medicine

Presentation(s):

Send Email for Thomas Vazquez

Daniela Frasca

Research Asst Prof
U Miami Miller School of Medicine

Presentation(s):

Send Email for Daniela Frasca


Assets

Molecular and Cellular Mechanisms for B Cell Defects in Elderly and Obese Subjects



Attendees who have favorited this

Send Email for Molecular and Cellular Mechanisms for B Cell Defects in Elderly and Obese Subjects