Immunity & infection

Oral Abstract Sessions

Comparison of IgA+ Plasma Cells in the Intestinal Mucosa Compartment versus the Periphery in a Healthy Kenyan Cohort

Thursday, June 15
3:39 PM - 3:52 PM

Little is known about the function of B cell lineages in the human intestinal mucosa, an important effector site for activated immune cells. Since cellular readouts on the peripheral blood mononuclear cells may not reflect the cellular phenotypes within the intestine, we analyzed IgA+ plasma cells (PCs), the main B cell subset of the intestinal lamina propria, in a healthy cohort of Kenyans with the goal of determining whether IgA+PCs correlate with T helper cell subtypes in the intestinal mucosa versus in the peripheral blood. Peripheral blood, terminal ileum (TI) and sigmoid colon (SC) biopsies from healthy individuals under colonoscopy examination were collected and studied by flow cytometry. Compared with peripheral blood and TI, SC was found to be highly enriched in PCs, the majority of which were IgA+PCs. We also observed that the frequency of IgA+PC positively correlated with interleukin (IL)-17, interferon g, IL-22, and tumor necrosis factor a-producing CD4+ T cells in the TI, but not in the SC or the peripheral blood. These results suggest that 1) in the SC and the peripheral blood, the T helper cell subsets we examined were not directly associated with the frequency of IgA+ PCs; 2) in the TI, multiple cytokines produced by CD4+ T cells have a significant impact on the abundance of IgA+ PCs. Our next steps are to look at specific factors produced by IgA+PCs including IL-10, Granzyme B and inducible nitric oxide synthase by immunofluorescence, and to correlate our FACS and immunofluorescence findings with gut permeability measurements.

Tian Sun

Graduate Student
University of Toronto, Dept of Immunology

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Xingyan Wang

Graduate student
University of Toronto, Dept of Immunology

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Olga L. Rojas

Research Associate
University of Toronto, Dept of Immunology

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Jun Liu

Post doc
University of Toronto, Dept of Immunology

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Akiso M. Mbendo

University of Nairobi, KAVI Institute of Clinical Research

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Simon Ogola

University of Nairobi, KAVI Institute of Clinical Research

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Brian Onsembe

University of Nairobi, KAVI Institute of Clinical Research

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Patrick M. Jeremiah

University of Nairobi, KAVI Institute of Clinical Research

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Bashir Farah

University of Nairobi, KAVI Institute of Clinical Research

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Gary Chao

University of Toronto, Dept of Immunology

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Omu Anzala

University of Nairobi, KAVI Institute of Clinical Research

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Rupert Kaul

Professor
University of Toronto, Dept of Immunology

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Mario Ostrowski

Professor
University of Toronto, Dept of Immunology

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Jennifer L. Gommerman

Professor
University of Toronto, Dept of Immunology

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Comparison of IgA+ Plasma Cells in the Intestinal Mucosa Compartment versus the Periphery in a Healthy Kenyan Cohort



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