Moderated Poster

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MP41-02: Prophylaxis against renal ischemia reperfusion injury in canine model: stem cell approach.

Saturday, May 13
3:30 PM - 5:30 PM
Location: BCEC: Room 156

Presentation Authors: Yasser Osman*, Sahar M. Hamed, Nashwa Barakat, Sherry Khater, Mahmoud Gabr, Ahmed Mosbah, Mohamed Gaballah, Atallah Shaaban, Mansoura, Egypt

Introduction: To date, there is no evidence exploring the protective effects of stem cells on minimizing ischemia reperfusion injury in a higher animal model.

Methods: A group of 60 dogs were maintained to perform kidney injury model. Ischemia of 90 minutes were performed by open occlusion of left renal artery in 57dogs. Group I included 27 dogs that were treated with Bone marrow derived Mesenchymal Stem cells (BM-MSC) while group II (27 dogs) treated with adipose tissue derived Mesenchymal Stem cells (AT-MSC). Each group was divided into 3 subgroups (9 dogs each), according to the stem cell dose (5, 10, 15 X 10 in 500 µl volume) injected directly in the kidney cortex after reperfusion. 3 dogs were positive control without MSC treatment while 3 were sham-operated. All dogs were re-evaluated by renogram prior to sacrifice at 2 weeks, 2 and 3 months as well as histopathology of the investigated kidney. Pro-inflammatory markers CD95 and TNF were assessed using real time PCR.

Results: In group I, there was mean reduction of clearance of the investigated kidney by a mean of 78%,64% and 74% of the three used doses respectively at 2 weeks. At 3 months, these kidneys regained a mean of 84%, 92% and 72% respectively of its basal function. In group II, the reduction of clearance was much more modest with mean of 14%, 6% and 24% respectively at 2 weeks with more intense recovery of renal function by mean of 90%,100% and 76% respectively. Positive control showed more intense reduction of clearance by 90% at 2 weeks that regained 70% of basal function at 3 months. On histopathologic examination in group I, there was more significant tubular necrosis and delayed regeneration compared with group II. The histopathological insult was more pronounced in the control group with more delayed recoverability. Expressions of Pro-inflammatory markers were up regulated in both groups with higher and more sustained expression observed in group I.

Conclusions: Stem cell might protect against the ischemia reperfusion injury in canine model. AT-MSC would provide the best protective potentiality compared with BM-MSC.

Source Of Funding: This project was supported financially by the Science and Technology Development Fund (STDF), Egypt, Grant No 4713)

Yasser M. Osman

Mansoura Urology & Nephrology Center

Dr Yasser Osman is a professor of Urology working in the Urology and Nephrology Center in Mansoura University. Being a staff member of the urology department; he shares in the clinical as well as academic activities for the last 20 years He started his clinical career by joining the center as a resident in urology in 1994. After finishing his master degree in urology in 1998, he received the title of assistant lecturer in urology. In year 2003, he presented his thesis in the field of renal transplantation to be assigned as a lecturer in urology. In this peroid, dr Osman visited Mayo Clinic, Cleveland Clinic as well as the Memorial Sloan-Kettering Cancer Center in USA as an international scholar. Currently, he is a professor of urology since July 2013. He is also is invited as a renal transplant consultant surgeon in major hospitals in KSA and Libya. Over the past years, Dr Osman shared in more than 100 local and international meeting presenting more than 95 abstracts covering different fields of urology focusing upon the fields of renal transplantation, uro-oncology as well as animal experimentation. He shared in the publication of 65 scientific papers in peer reviewed scientific International journals and shared in the authorship of 3 book chapters. Moreover, Dr Osman is an international reviewer in 12 different international scientific journals in the fields of urology and renal transplantation and he was invited to be an invited guest speaker in several meetings in Europe, Asia & Africa. Dr Osman also shared and directed 15 workshops inside and outside the country by lecturing, performing live surgery and guiding animal laboratory surgeries. Finally Dr Osman is a member of the Egyptian, American as well as the European urological associations and he was honored by receiving 8 different national and international prizes .

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MP41-02: Prophylaxis against renal ischemia reperfusion injury in canine model: stem cell approach.



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