Poster, Podium & Video Sessions
Presentation Authors: Yuke Chen*, Wei Yu, Yang Yang, Shiliang Wu, Jie Jin, Beijing, China, People's Republic of
Introduction: To explore the function of inflammatory regulation by PD-L1 on the onset and progression of interstitial cystitis (IC).
Methods: The clinical data of IC patients underwent hydrodistension (HD) plus bladder biopsy from 2009 to 2016 at our center were reviewed. The IC was diagnosed as: 1.pressure or pain at bladder accompanied by frequency and nocturia ≥ six weeks; 2. glomerulation or Hunner's ulcer on cystoscopy; 3. exclusion of other disease by pathology. The UPOINT scoring (Urinary symptom, Psychosocial symptom, Organ-specific symptom, Infection, Neurologic symptom and Tenderness) was assessed. HD outcomes were evaluated according to global response assessment (GRA) and UPOINT at postoperative 3 mo. The inflammation degree was semi-quantitatively assessed on Haematoxylin and eosin (H&E) staining. The immunohistochemistry (IHC) for PD-L1 expression dection and immunofluorescence for T cell and B cell counting were performed.
Results: The present study includes 8 males and 32 females with median age of 57 years. The inflammation degree of the bladder wall on H&E is negatively correlated with disease course (P=0.026) and positively correlated with the bladder pain degree (P<0.001). HD is effective at postoperative 3 mo in 19 subjects (GRA≥2), whose UPOINT scores indicate a highest relief rate of the bladder pain (89.5%, 17/19) followed by urinary symptoms (52.6%, 10/19). There are 17, 15, 7 and 1 subjects having none, mild, moderate and high degree of PD-L1 expression (fig. A). And PD-L1 expression degree is correlated with H&E inflammation degree and T cell count (fig. B). Further, PD-L1 expression degree and T cell count are positively correlated with the bladder pain degree (fig. B). Among the 12 subjects with severe inflammation on H&E, HD is more effective for subjects with moderate or high PD-L1 expression (83.3% vs. 16.7%, P=0.04) (fig. C).And subjects with moderate or high PD-L1 expression tended to have more effective hydrodistension outcome than subjects without PD-L1 expression (7/8 vs. 7/17, P=0.038).
Conclusions: PD-L1 expression is more common in IC patients with serious bladder pain and severe bladder inflammation. IC patients with higher degree of PD-L1 expression tend to have more effective HD outcome.
Source Of Funding: This work was supported by Grants from the National Natural Science Foundation of China to (Granted No. 81200548).
Name: Yuke Chen.
Degree: resident, medical doctor in urology.
City and Country: Beijing, China.
Department and affiliation: Department of Urology, Peking University First Hospital.
I am a urologist from China. My domain is neurourogy and urodynamics. It is honorable to present and share some of important results of my recent basic study in interstitial cystitis.