Moderated Poster

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MP94-16: Oxidative stress-related alterations in the bladder of a short-period diabetes type-2 rat model.

Tuesday, May 16
7:00 AM - 9:00 AM
Location: BCEC: Room 253AB

Presentation Authors: Panagiota Tsounapi*, Masashi Honda, Yonago, Japan, Fotios Dimitriadis, Ioannina, Greece, Yusuke Kimura, Yonago, Japan, Shogo Shimizu, Nankoku, Japan, Bunya Kawamoto, Katsuya Hikita, Yonago, Japan, Motoaki Saito, Nankoku, Japan, Nikolaos Sofikitis, Ioannin

Introduction: Diabetes type-2 accounts for almost 90% of diabetes cases worldwide. Diabetes among other complications induces bladder dysfunction. In the current study we aimed to create a short-period diabetes type-2 model in order to investigate oxidative stress-related alterations in the bladder in the initiation of the disease. Additionally antioxidant treatment with resveratrol or taurine was provided in order to examine whether it is possible to prevent these alterations in the very beginning of the disease.

Methods: Diabetes was induced in 8-week-old male Wistar rats with a single dose of streptozotocin (40mg/kg) intraperitoneally (i.p.). The next day they were randomly separated into 3 groups and 14 days feeding with a high fat diet followed. One group received no treatment (DM group), another group received orally resveratrol (10mg/Kg; Resv group) and the third one received taurine i.p. (1g/Kg; Tau group). Age matched control animals were used and were fed with normal diet (Control group). Two weeks later animals were sacrificed and bladder were processed for histological evaluation, measurements of malondialdehyde (MDA) and immunohistochemistry (IHC) for oxidative stress markers.

Results: At the end of 2 weeks all diabetic groups had significantly lower body weight compared to the Control group. DM group demonstrated significantly higher ratio of bladder weight to body weight compared to the Control (Figure 1). Histological evaluation demonstrated mild damage of the bladder tissue in the DM group such as abruption of the mucosa from the muscularis as well as edema in the transitional epithelium. All these alterations were not observed in the treatment groups. MDA levels in the bladder were significantly larger in the DM group compared with Control, Resv or Tau group (Figure 1). Fourteen days of diabetes without treatment in the DM group induced moderate to strong intensity and positivity for oxidative stress marker MDA, 4-Hydroxynonenal and DNA oxidative stress marker 8-deoxyguanosine compared with the other three groups.

Conclusions: The prompt diagnosis of diabetes can be crucial for the progression of the disease. Specifically in the bladder, it appears that both mild damage in structural level as well as oxidative damage in molecular level can be prevented by resveratrol or taurine treatment.

Source Of Funding: None

Panagiota Tsounapi, PhD

Tottori University Faculty of Medicine

Panagiota Tsounapi since April 2017 is a project researcher at the Diviison of Urology of Tottori University Faculty of Medicine.
Since July 2015 up to March 2017 she was an assistant professor in the Division of Urology of Tottori University Faculty of Medicine in Japan. Previous to that (April 2013−June 2015) she has been selected as a Postdoctoral Foreigner Fellow of the Japan Society for the Promotion of Science. In March 2013 she graduated the PhD course of the Graduate School of Medical Sciences of Tottori University under a scholarship of the Japanese Ministry of Education, Culture, Sports, Science and Technology for international students. She has graduated the Department of Biological Applications and Technologies of University of Ioannina, Greece.
Her research efforts focus on the oxidative stress−induced male infertility under pathological situations such as the diabetes, cryptorchidism, testicular torsion, etc.
At the same time she is working to obtain her second PhD from the Graduate School of Medicine of University of Ioannina, Greece, which she has paused in order to move to Japan.

For more information on her publication ist please follow the links below:
https://www.ncbi.nlm.nih.gov/pubmed/?term=tsounapi
https://www.ncbi.nlm.nih.gov/pubmed/?term=panagiota+t

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