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MP94-01: Protective effect of platelet-rich plasma on urethral stricture model of male rats

Tuesday, May 16
7:00 AM - 9:00 AM
Location: BCEC: Room 253AB

Presentation Authors: HASAN TAVUKCU, OMER AYTAÇ, FATİH ATUĞ, BURÇİN ALEV, ISTANBUL, Turkey, OZGE CEVİK, Sivas, Turkey, AYŞEN YARAT, SULE CETİNEL, GÖKSEL SENER, HALUK KULAKSIZOĞLU*, ISTANBUL, Turkey

Introduction: Urethral stricture formation is caused by fibrosis after excessive collagen formation to the any injury or trauma to the urethra. We evaluated the affects of platelet-rich plasma (PRP) on urethral stricture model of male rats

Methods: Urethral stricture model was performed by coagulation current to the male urethra. There were 4 groups of 6 rats in each one: control (C), C+PRP applied, urethral stricture (US), US+PRP applied. PRP was applied to the urethra after coagulation current induced injury as soon as possible. On 14th day all rats were sacrified and urethral tissues investigated for collagen type I (col I), collagen type III (col III), platelet-derived growth factor-A (PDGF-A), platelet-derived growth factor-B (PDGF-B) and transforming growth factor-B (TGF-B) with quantative real-time polymerase chain reaction (RT-PCR) and Western-Blot analysis. The efffect of urethral damage and healing was investigated for col I/col III ratio.

Results: Collagen type I increase in fibrosis process in US is well defined. Collagen type I/ collagen type III ratio was significantly high in US group (*, p=0,000) than others while US+PRP group had comparable results with the control group (p=0,999). (Graphic 1) RT-PCR analysis of col I, col III, PDGF-A, PDGF-B and TGF-B was shown in table 1.
Graphic 1: Col I/Col III type ratio of each group.
Table 1: Results of RT-PCR analysis of col I, col III, PDGF-A, PDGF-B and TGF-B.(* Significant between US and US+PRP groups; & not significant between C and US+PRP groups; ** significant between C and US+PRP groups; )





Conclusions: Our results show that PRP has a preventive effect on stricture formation in US rat model shown by its effect on collagen synthesis, especially in recurrent cases. Further studies that eventually show the effects of PRP on human tissues is necessary and promising.

Source Of Funding: Istanbul Bilim University

HALUK KULAKSIZOĞLU, professor in urology

Istanbul Bilim University

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