Moderated Poster

Poster, Podium & Video Sessions

MP11-06: Expression of Inflammatory Mediators in Sensory Ganglia Innervating Lower Urinary Tract And Dysfunctional Voiding

Friday, May 12
1:00 PM - 3:00 PM
Location: BCEC: Room 151

Presentation Authors: Pradeep Tyagi*, Mahendra Kashyap, Zhou Wang, Naoki Yoshimura, Pittsburgh, PA

Introduction: Dysfunctional voiding associated with chronic prostatic inflammation
is considered to be caused by sensitization of primary sensory neurons innervating lower urinary tract. Published research implicates pro-inflammatory cytokines as major player in the neural sensitization and the progression of prostatitis. Therefore, we investigated the expression of cytokines and chemokines in dorsal root ganglia (DRG) from the lumbosacral region in rat model of non-bacterial prostatitis.

Methods: Intraprostatic injection of formalin (50μL) or saline (sham) was performed in three month- old male Sprague-Dawley rats to induce prostatitis(n=3). 12 hour night time urination pattern were noted a day before injection and 7 days later. Lumbosacral L6-S1 and cervical (C4) DRG were isolated from the sacrificed animals on the 7th day for multiplex analysis of 27 cytokines, chemokines and growth factors using a MILLIPLEX MAP Rat Cytokine/Chemokine Panel kit. Results are expressed as pg/mg of total protein

Results: The expression of IFN-γ, CXCL-10, VEGF and EGF was signficantly elevated in the L6-S1 DRG relative to the C4 DRG of either group(*p<0.05). Expression of CXC chemokines (CXCL-1, CXCL-2, CXCL-5), CC chemokines (CCL2, CCL3, CCL5), leptin, IL-2, IL-13 and IL-17A was only elevated in L6-S1 DRG relative to C4 DRG of sham group (#p<0.05). Frequent urination and reduced voided volume in the prostatitis group was also associated with substantial but insignficant increase in the production of CXCL-1 (p=0.06), CCL2 and leptin in the C4 DRG relative to that of the sham group.


Conclusions: Dysfunctional voiding secondary to prostatic inflammation was linked to the dramatic overproduction of inflammatory mediators in L6-S1 DRG capable of inducing phenotypic changes in micturition reflex pathways. Since cervical DRG is not directly innervated by the axons of afferent neurons from prostate and bladder, therefore, sustantial production of leptin, CXCL-1 and CCL2 in the cervical DRG may be humorally mediated in prostatitis to suggest a role for neurohumoral interaction in the evolution of prostatitis into a regional pain syndrome.

Source Of Funding: NIDDK, U54 DK112079

Pradeep Tyagi, PhD

University of Pittsburgh

Pradeep Tyagi, Ph.D. is the Associate Professor in the urology Department at University of Pittsburgh. His current research is on the role of neurotrophins and microRNA in voiding disorders, drug development and functional proteomics in lower urinary tract. He has published over 100 research articles and his research has been recognized at American Urological Association and Society of Urodynamics and Female Urology.

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MP11-06: Expression of Inflammatory Mediators in Sensory Ganglia Innervating Lower Urinary Tract And Dysfunctional Voiding



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